Ashlar is the tool for turning individual tile images from all of your imaging cycles into a single large multi-channel image:
mkdir my_project
cd my_project
ashlar /path/to/cycle1_image /path/to/cycle2_image ...
This will leave you with a series of full-size TIFF files named cycle_A_B.tif
where A
is the cycle number and B
is the
channel number (both starting with 0). The input image files can be any multi-series imaging file with stage positions that
BioFormats can read. RareCyte .rcnpnl
and InCell .xdce
files have both been verified to work.