ag1805x

#Extract gene ids of particular biotype from GFF3 files
#Assuming: GFF3 file is Homo_sapiens.GRCh38.84.gff3 (ftp://ftp.ensembl.org/pub/release-84/gff3/homo_sapiens/Homo_sapiens.GRCh38.84.gff3.gz)
#Assuming: biotype=protein_coding

grep "biotype=protein_coding" Homo_sapiens.GRCh38.84.gff3 | cut -f9 | cut -d';' -f1 |cut -d'=' -f2 | grep "gene" | cut -d':' -f2 | sort | uniq > proten_coding_ids

ag1805x

#Using NCBI's efetch to retrive GSM id (sample id) from a given SRR id(run id)

efetch -db sra -id SRR2453355 -format xml | xmllint --xpath "EXPERIMENT_PACKAGE_SET/EXPERIMENT_PACKAGE/EXPERIMENT/@alias" - | cut -d \" -f2

ag1805x

#Code to cdeate heatmap of riboswitch counts in different bacteria.


library(pheatmap)
data<- as.matrix(read.csv("heat_map_table.csv", header=T, row.names=c(1)))
col_key = c("#800000", "#e6194B", "#3cb44b", "#ffe119", "#4363d8", "#f58231", "#911eb4", "#42d4f4", "#f032e6", "#bfef45", "#fabebe", "#469990", "#fffac8")
#Since max count was 12 we used 12 distinct colours
pheatmap(data, treeheight_row = 0, treeheight_col = 0, cluster_rows=FALSE, cluster_cols=FALSE, color=rev(col_key), cellwidth=16, cellheight=15, height=15, width=10,filename="Heatmap.png")

ag1805x

#!/usr/bin/env bash
# make_rRNA.sh
# Kamil Slowikowski
# December 12, 2014
#
# Modified: Arindam Ghosh (July 24, 2019 )
#
#
# Referenc Genome: GRCh38.p5 Ensembl release 84
#

ag1805x

###########################################
# Code to extract GC% of genes from GFF file
# using bedtools
# - Arindam Ghosh (22 August 2019)
###########################################


bedtools nuc -fi Homo_sapiens.GRCh38.dna.primary_assembly.fa -bed Homo_sapiens.GRCh38.84.gff3 | grep ID=gene:ENSG > temp.txt

echo -e "GeneID\tpct_GC" > GRCh38_GeneGC.txt 

ag1805x

library(dendextend)

countsPC <- read.table("CountsPC_Gene_MultimapOverlap.tsv", header=T, row.names=c(1))
factors <- as.data.frame(read.table("factors.txt", sep="\t", header = TRUE, row.names=c(1)))

dist <- dist(t(countsPC), method="euclidean")
cluster <- hclust(dist)


# main, sub, xlab, ylab

ag1805x

# Code to generate tx2gene from reference transcriptome for use with tximport() in R
# Reference transcriptome: wget ftp://ftp.ensembl.org/pub/release-97/fasta/homo_sapiens/cdna/Homo_sapiens.GRCh38.cdna.all.fa.gz

 
zcat Homo_sapiens.GRCh38.cdna.all.fa.gz | grep '>' | cut -d ' ' -f1,4,7 > temp
paste <(cut -d '>' -f2 temp | cut -d ' ' -f1) <(cut -d ' ' -f2 temp | cut -d ':' -f2) <(cut -d ' ' -f3 temp | cut -d ':' -f2) >> tx2gene.txt
rm temp

ag1805x

# The PANTHER GO output doesnot directly support visualisation by REVIGO. 
# Using this single line code in linux terminal we can make it compatible for REVIGO
# Gene Ontology enrichment analysis done using PANTHER (interface from: http://geneontology.org/)
# The results are exported as Table
# The initial few lines are cleaned to keep only the result table on which we execute the following:

paste <(cut -f1 PantherOut.tsv | cut -d "(" -f2 | cut -d ")" -f1) <(cut -f7 List.tsv)  >> RevigoIn.tsv

ag1805x

# Extract list of unique Gene IDs in GTF file

awk '{if($3 == "gene") print $0}' ../Homo_sapiens.GRCh38.84.gtf | cut -f9 | cut -d ';' -f1 | cut -d ' ' -f2 | sort | uniq | wc -l

# Extract gene list of particular biotype

grep 'gene_biotype "protein_coding"' ../Homo_sapiens.GRCh38.84.gtf | awk '{if($3 == "gene") print $0}' | cut -f9 | cut -d ';' -f1 | cut -d ' ' -f2 | sort | uniq | wc -l

# Create subset of Ensembl GTF file based on gene biotype

ag1805x

# Extract mature and precursor miRNA list from miRBase gff3 file
# Can be used for interconversion

grep -v '#'  hsa.gff3 |  grep -w "miRNA" | cut -f9 > temp
paste <(cut -d ';' -f1 temp | cut -d '=' -f1) <(cut -d ';' -f2 temp | cut -d '=' -f1) <(cut -d ';' -f3 temp | cut -d '=' -f1) <(cut -d ';' -f4 temp | cut -d '=' -f1) | head -n1 >> miRbaseIDs.tsv
paste <(cut -d ';' -f1 temp | cut -d '=' -f2) <(cut -d ';' -f2 temp | cut -d '=' -f2) <(cut -d ';' -f3 temp | cut -d '=' -f2) <(cut -d ';' -f4 temp | cut -d '=' -f2) >> miRbaseIDs.tsv