Launch an AWS EC2 instance.
Install Miniconda:
wget https://repo.anaconda.com/miniconda/Miniconda3-py38_4.10.3-Linux-x86_64.sh
bash Miniconda3-py38_4.10.3-Linux-x86_64.sh
conda create -n pysam python=3.6.5 pip
sudo yum install -y openssl-devel
wget https://github.com/pysam-developers/pysam/archive/v0.15.3.tar.gz
tar xvzf v0.15.3.tar.gz
cd pysam-0.15.3
sudo yum install git autoconf automake make gcc zlib-devel bzip2-devel xz-devel curl-devel openssl-devel
git clone https://github.com/samtools/htslib.git
cd htslib/
autoconf
autoheader
./configure
make
make test
sudo make install
conda install -c bioconda samtools=1.9=h8ee4bcc_1 openssl=1.0
from IPython.core.display import display, HTML
display(HTML("<style>.container { width:95% !important; }</style>"))
Flags:
0x1 PAIRED .. paired-end (or multiple-segment) sequencing technology
0x2 PROPER_PAIR .. each segment properly aligned according to the aligner
0x4 UNMAP .. segment unmapped
0x8 MUNMAP .. next segment in the template unmapped
0x10 REVERSE .. SEQ is reverse complemented
0x20 MREVERSE .. SEQ of the next segment in the template is reversed
0x40 READ1 .. the first segment in the template
0x80 READ2 .. the last segment in the template
Download the package: http://www2.futureware.at/~nickoe/msys2-mirror/msys/x86_64/rsync-3.1.2-2-x86_64.pkg.tar.xz
Extract it and move rsync.exe
to %HOMEDRIVE%%HOMEPATH%\AppData\Local\Programs\Git\usr\bin
.
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