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#load everythin in memory | |
#split | |
def split_fasta(input_file): | |
with open(input_file) as fasta_file: | |
text = fasta_file.read().split(">")[1:] | |
data = [] | |
for entry in text: | |
header,sequence = entry.split("\n",1) | |
sequence = sequence.replace("\n","") |
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sudo ntpd -gq | |
sudo hwclock --systohc --utc |
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#!/usr/bin/env python | |
#http://rosalind.info/problems/cons/ | |
import fastaparse | |
letters = ["A","C","G","T"] | |
sequences = [i.seq for i in fastaparse.sequences("temp2")] | |
#create empty profile matrix |
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#!/usr/bin/env perl | |
use v5.14; | |
use warnings; | |
sub hamming { | |
my @a = split "", shift; | |
my @b = split "", shift; | |
my $distance = 0; | |
for (my $n = 0; $n < scalar(@a); $n++) { | |
unless (($a[$n]) eq ($b[$n])) { |
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join -a 1 -a2 -e 'NA' -o '0,1.2,2.2' ce hc > join.count |
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tail -n+4 -q results/* | less -S |
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^\<\u\l\{-}\> \<\l\{-}\>$ |
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# blosum62 | |
# * column uses minimum score | |
# BLOSUM Clustered Scoring Matrix in 1/2 Bit Units | |
# Blocks Database = /data/blocks_5.0/blocks.dat | |
# Cluster Percentage: >= 62 | |
# Entropy = 0.6979, Expected = -0.5209 | |
A R N D C Q E G H I L K M F P S T W Y V B Z X * | |
A 4 -1 -2 -2 0 -1 -1 0 -2 -1 -1 -1 -1 -2 -1 1 0 -3 -2 0 -2 -1 0 -4 | |
R -1 5 0 -2 -3 1 0 -2 0 -3 -2 2 -1 -3 -2 -1 -1 -3 -2 -3 -1 0 -1 -4 | |
N -2 0 6 1 -3 0 0 0 1 -3 -3 0 -2 -3 -2 1 0 -4 -2 -3 3 0 -1 -4 |
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#Take a fraction of random sequence reads from a fastq file | |
from sh import wc | |
import argparse | |
import random | |
def first_word(string): | |
return string.strip().split()[0] | |
#read a file in chunks of deflines | |
def read_segments(filename,deflines): |
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#!/bin/sh | |
cmd=$* | |
pid=$$ | |
echo "$cmd @ `pwd`; Started at `date`" > $pid.out | |
echo "[$pid] $cmd" | |
eval $cmd >> $pid.out 2>> $pid.err | |
echo "$cmd @ `pwd`; Finished at `date`" >> $pid.out |