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saveas( handles.axes2 , 'D:/maj proj/samples/output.jpg' );

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F = getframe(handles.axes2);
Image = frame2im(F);
imwrite(Image,'D:/folder/output.jpg','jpg');

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          if (strcmp('a','radiobutton1')
            imshow(c,'Parent',handles.axes2);

   
          elseif (strcmp('b','radiobutton2')) 
                  imshow(b,'Parent',handles.axes2);     
          end

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function pushbutton1_Callback(hObject, eventdata, handles)


a=get(handles.edit1, 'string');
 b=imread(a);
 c=rgb2gray(b);
 imshow(c,'Parent',handles.axes2);

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image=get(handles.edit1, 'string');

imshow(image,'Parent',handles.axes2);

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imgurl = strcat(pn,fn);
set(handles.edit1,'string',imgurl);
I = imread(imgurl);
imshow(I,'Parent',handles.axes1);
guidata(hObject, handles);

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function pushbutton2_Callback(hObject, eventdata, handles)
% above line of code is default line ofcode for button



% an example to open image files only 
% when button is clicked, a dialog box appears to select the file
handles.output = hObject;
[fn pn] = uigetfile( {  
   '*.png;*.jpg;*.jpeg;*.gif','Image Files (*.png, *.jpg, *.jpeg, *.gif)'; ...

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% --- Executes on button press in pushbutton2.
function pushbutton2_Callback(hObject, eventdata, handles)


handles.output = hObject;
[fn pn] = uigetfile('*.png','select png file');
complete = strcat(pn,fn);
set(handles.edit1,'string',complete);
I = imread(complete);
imshow(I,[]);

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% code by juniorhero to show image in axes in gui
axes(handles.axes2);
imshow('D:\maj proj\samples\Lenna.png')

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a=imread('Koala.jpg');
b=255-a
subplot(1,2,1)
imshow(a)
title 'Original Image'
subplot(1,2,2)
imshow(b)
title 'Negative Image'