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May 3, 2011 21:31
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waaronw's sequencing results for TAS2R38 SNP rs713598
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| >AF9849-Aaron_TAS2R_test-rs713598_For_A12.ab1 | |
| NNNNNNNNNNNCNTGTCTCNTGTGCCTTCCCAGGGAGACAGTCAGCATCCCAGAAGAAACCAGAAACAATAGGAAAGGAG | |
| GCACAGACCACAGATAGCAGAAGAGAAAGGAATAAAATAAATTGAGATCTTTAATCTGCCAGTTGAGCCTTGTATTGTTA | |
| TTCATGAATAGCACAGTTGTGACTGTGAAGTGAGGTCTGCTAAAAAAGCACCAAACACAGAGGACAGTGCAGATGCAGGA | |
| GCAAAGAATAATACCCAGGAGCATCTGGGAGATCTTCCTGGAGACCCAGCTTGCCAAGCAGATCAGGAAGGTGTGAGAGA | |
| AACGGATGAGCTTGGAGCAGTAAAGCAGGCTGAGGCAGGCAGCAAGCCAGAGGTTGGCTTGGTTTGCAATCATCCATAGC | |
| ATGATGATGGCTTGGTAGCTGTGGTTCAGTGGTTCACTCAACTTCTGGAAGTGGGTAAGCTGGATAGCACTCAGGAACAG | |
| CAGTCCATGCAGGAAAAGCCGGCTGATGCTGAGACACAGCAGCACACAATCACTGTTGCTCAGTGCCTGCCTCTTCACTA | |
| CATCCCAAAAATTCACCAAGAAAACGAAGGCATTGGTCAGAAACCCCACTGCAAACTCCAGGACTGAAATGAACAGAAAT | |
| GTACTCCTGACTTCATAGGACACAGTGCGGATGCGAGTTAGAGTCAACATGATGTCACTTCTCTAATTGGCTATTCTACT | |
| TCTCTTCTCTAGTTGGCTAATCTAAAGACCTGGTTGCCACCCAGTGCAGAAAGGTAAATGTACGTTCCAGCCNGNNGNNN | |
| NNNNNNNNNANN |
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| rs713598 - Bitter Taste (TAS2R38) | |
| ================================= | |
| http://www.ncbi.nlm.nih.gov/gene/5726 | |
| NG_016141.1 | |
| *Updated Gene Map w/ Primers*: | |
| - http://tinyurl.com/23fhf36 | |
| Primer Sequences (~833 bp amplicon) | |
| - TCCAGGCTGGGGTCACGAGAG (Forward Primer) | |
| - GGAAAGGTCCTGGCTTGGAACGTA (Reverse Primer) | |
| - used forward primer as sequencing primer | |
| - *note:* based on http://bioinformatics.dnalc.org/ptc/animation/index.html | |
| BLAST SEARCH RESULTS AGAINST PRIMERS | |
| ------------------------------------ | |
| >ref|NG_016141.1| Homo sapiens taste receptor, type 2, member 38 (TAS2R38), RefSeqGene | |
| on chromosome 7 | |
| Length=8143 | |
| Score = 48.1 bits (24), Expect = 0.002 | |
| Identities = 24/24 (100%), Gaps = 0/24 (0%) | |
| Strand=Plus/Plus | |
| Query 22 GGAAAGGTCCTGGCTTGGAACGTA 45 | |
| |||||||||||||||||||||||| | |
| Sbjct 4971 GGAAAGGTCCTGGCTTGGAACGTA 4994 | |
| Score = 42.1 bits (21), Expect = 0.094 | |
| Identities = 21/21 (100%), Gaps = 0/21 (0%) | |
| Strand=Plus/Minus | |
| Query 1 TCCAGGCTGGGGTCACGAGAG 21 | |
| ||||||||||||||||||||| | |
| Sbjct 5803 TCCAGGCTGGGGTCACGAGAG 5783 |
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