readme.md

see the output of the notebook here: http://nbviewer.jupyter.org/gist/100ideas/ac4d39e1fd10824459a941e4c653f8b9/coral-d3-plasmid.ipynb

sources:

• pMODKan-HO-pACT1GEV.ape
• coral d3-plasmid.js

process:

• spend way too long figuring out undocumented config problems with "official" coral dockerhub image
  • (dockerfile is missing; docker image 'cmd' doesn't launch jupyter automatically; coral is out of date; not configured to allow connections except localhost; bleh. just make a new dockerfile next time)
• clone klavinslab/coral for tutorial files local to jupyter
• run local jupyter notebook (python 2.7 kernel)

     python2 -m ipykernel install
     -m pip install ipykernel --upgrade
  

• commands as below

coral-d3-plasmid.ipynb

plasmid.d3.ipynb.md

import coral as cor

pKL278 = cor.seqio.read_dna('files_for_tutorial/maps/pMODKan-HO-pACT1GEV.ape')

pKL278.display()

pMODKan-HO-pACT1GEV.ape.json

{
  "features": [
    {
      "color": [
        "#96bbff"
      ],
      "start": 28,
      "stop": 51,
      "name": "pGEX_3_primer",
      "type": "misc_feature"
    },
    {
      "color": [
        "#0064ff"
      ],
      "start": 132,
      "stop": 154,
      "name": "pMOD_t1pre",
      "type": "misc_feature"
    },
    {
      "color": [
        "#ff0000"
      ],
      "start": 154,
      "stop": 162,
      "name": "PmeI(1)",
      "type": "misc_feature"
    },
    {
      "color": [
        "cyan"
      ],
      "start": 162,
      "stop": 725,
      "name": "HO Targeting 1",
      "type": "misc_feature"
    },
    {
      "color": [
        "#0071ff"
      ],
      "start": 725,
      "stop": 755,
      "name": "pMOD_t1suf",
      "type": "misc_feature"
    },
    {
      "color": [
        "#edd079"
      ],
      "start": 755,
      "stop": 1152,
      "name": "KANMX Wach et al 1994 (genome del. project)",
      "type": "misc_feature"
    },
    {
      "color": [
        "#acb1ff"
      ],
      "start": 1152,
      "stop": 1962,
      "name": "KanMX CDS",
      "type": "misc_feature"
    },
    {
      "color": [
        "#e9c021"
      ],
      "start": 1962,
      "stop": 2200,
      "name": "KanMX terminator",
      "type": "misc_feature"
    },
    {
      "color": [
        "#1ed628"
      ],
      "start": 2200,
      "stop": 2224,
      "name": "M13 Forward (-47) primer",
      "type": "primer_bind"
    },
    {
      "color": [
        "#f6ff39"
      ],
      "start": 2224,
      "stop": 2885,
      "name": "pACT1",
      "type": "misc_feature"
    },
    {
      "color": [
        "cyan"
      ],
      "start": 2921,
      "stop": 2932,
      "name": "Extra sequence not found in Gottschling map",
      "type": "misc_feature"
    },
    {
      "color": [
        "#00ff29"
      ],
      "start": 2940,
      "stop": 3218,
      "name": "GAL4(1-93) DBD",
      "type": "misc_feature"
    },
    {
      "color": [
        "cyan"
      ],
      "start": 3218,
      "stop": 3219,
      "name": "Differs from Gottschling map (backbone)",
      "type": "misc_feature"
    },
    {
      "color": [
        "#6c59ff"
      ],
      "start": 3255,
      "stop": 4140,
      "name": "hER HBD",
      "type": "misc_feature"
    },
    {
      "color": [
        "cyan"
      ],
      "start": 4140,
      "stop": 4344,
      "name": "HSV1 VP16",
      "type": "misc_feature"
    },
    {
      "color": [
        "#ff4638"
      ],
      "start": 4235,
      "stop": 4236,
      "name": "Differs from Gottschling Map",
      "type": "misc_feature"
    },
    {
      "color": [
        "#d0410e"
      ],
      "start": 4344,
      "stop": 4347,
      "name": "stop codon",
      "type": "misc_feature"
    },
    {
      "color": [
        "#40ff40"
      ],
      "start": 4347,
      "stop": 4377,
      "name": "L2",
      "type": "misc_feature"
    },
    {
      "color": [
        "#eb66ff"
      ],
      "start": 4377,
      "stop": 4399,
      "name": "T + pBluescript KS linker",
      "type": "misc_feature"
    },
    {
      "color": [
        "#59c0cb"
      ],
      "start": 4403,
      "stop": 4643,
      "name": "CYC1",
      "type": "terminator"
    },
    {
      "color": [
        "#6cc0cb"
      ],
      "start": 4412,
      "stop": 4431,
      "name": "pYESTrp_rev primer",
      "type": "primer_bind"
    },
    {
      "color": [
        "#6cc0cb"
      ],
      "start": 4643,
      "stop": 4665,
      "name": "T7 EEV primer",
      "type": "primer_bind"
    },
    {
      "color": [
        "cyan"
      ],
      "start": 4665,
      "stop": 5571,
      "name": "upstream HO targeting",
      "type": "misc_feature"
    },
    {
      "color": [
        "#96bbff"
      ],
      "start": 5571,
      "stop": 5579,
      "name": "PmeI",
      "type": "misc_feature"
    },
    {
      "color": [
        "#ff0000"
      ],
      "start": 5571,
      "stop": 5579,
      "name": "PmeI site",
      "type": "misc_feature"
    },
    {
      "color": [
        "#00ff00"
      ],
      "start": 5579,
      "stop": 5619,
      "name": "M13R",
      "type": "misc_feature"
    },
    {
      "color": [
        "cyan"
      ],
      "start": 5804,
      "stop": 5889,
      "name": "origin-extended",
      "type": "misc_feature"
    },
    {
      "color": [
        "#f1de49"
      ],
      "start": 5889,
      "stop": 6744,
      "name": "ori",
      "type": "misc_feature"
    },
    {
      "color": [
        "cyan"
      ],
      "start": 6426,
      "stop": 6427,
      "name": "is a g in normal maps.",
      "type": "misc_feature"
    },
    {
      "color": [
        "#fcabe4"
      ],
      "start": 6744,
      "stop": 7605,
      "name": "bla",
      "type": "misc_feature"
    },
    {
      "color": [
        "#f96061"
      ],
      "start": 7605,
      "stop": 7684,
      "name": "AmpR promoter",
      "type": "misc_feature"
    },
    {
      "color": [
        "cyan"
      ],
      "start": 7684,
      "stop": 7704,
      "name": "New Feature",
      "type": "misc_feature"
    }
  ],
  "bottom": "GACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGGTTTAAACAATTATCCTGGGCACGAGTGAAACAAAGCTAAAACCTTTATTTAGCATGGCCATTGAATGTAACAATTATATATATCGCAAGCACAAAAAATCAAGGAGAGAGAACTACCACTTTGTTCATGTGTACAATGTTCATTATCTCCATAAGCAAAAAAAAAAAATAGAAAACATATGCTATAAGGTTGATATTCTCACGAGTAAGCGGCACTTGCTACTTATTGACATTGCAGATTTTTGGCTACAGAAATAGTATATTAGAGATTATAATTGCTAATCAAATCAAAATATAAAATTAGTAAACCAAACCATTTATACCCTTCCTTAGTAGTTATGGATTGTTTTTTAATGATATTTCTGCAAACCAAAGAAAGATTGTTATCCAGATAGAATTTAGTTTTGATATTCATTTTTTTGTTGAAGATTGAACGCCATATCTGGGCCTCATAATTCAAAAGACGGTGCCATTATCGGTAGCGTTTCGCATTGTACTGGATTTCAGAAATTTCACAGTTGATGAATCGAAAAGAATGGTCTCATTGCAACACGTAAGGTTAAGATGTCCCTTTTTACCATTATAGGCAATAAATGAATCATAAAACGACCGTATACTGGTGAAATAGTAGGGAGAACGAGTACCTGTAGTAAAAAGTATAAATCATAGTTAATCGGGCAATGTCCCTCGATCAAGGAGTATTGTGTCATGTTCGAGACAAACGCCAACATTTTTGTTTCTTTTGGACAAATGTTGTTTGCATTTATGATCCGTTATATTTTGATCTAATGTAGAGTTGCACGTAGTTCTTACTGGCAAAGAAATCGATGCATACCAAAAAAGAATAAAGGTGATATTTGATCTTTACCGTTTAGTTCCAACGTAAAATTGTGCCTTTGGACTTAAAATGGCGTCGGGGCGGGGTTATTACGACATCAAATTAAAGCCTTCGAGCGTCCCAAAACCTTCTCAAGCAAGGTTTTCAGTATAATGTTACATGCGTACACGCGTCTGTACAGAAAAAAAAGAAAAATTTGAAATATAAATAACGTTCTTAATACTAACATAACTATAAAAAAATAAATAGGGACCTAGACTTCAGGTTGTCTAACTCCTTCCTTTTCGGTTAGAGCGGATGTGGGGGGAGGGCGTGAATGTAAGCGTGACATAACTAATTACATGACTCGAGGTCGACGGTATCAGGATCCGGATCCACTTCCGGAACCGGATCCCTACCCACCGTACTCGTCAATTCCAAGGGCATCGGTAAACATCTGCTCAAACTCGAAGTCGGCCATATCCAGAGCGCCGTAGGGGGCGGAGTCGTGGGGGGTAAATCCCGGACCCGGGGAATCCCCGTCCCCCAACATGTCCAGATCGAAATCGTCTAGCGCGTCGGCATGCGCCATCGCCACGTCCTCGCCGTCTAAGTGGAGCTCCGATGAAGTAGAGCCCGCAGTGGCCAAGTGGCTTTGGTCCGTCTCCTCCACGGATGCCCCTCCACGGCTAGTGGGCGCATGTAGGCGGTGGGCGTCCAGCATCTCCAGCAGCAGGTCATAGAGGGGCACCACGTTCTTGCACTTCATGCTGTACAGATGCTCCATGCCTTTGTTACTCATGTGCCTGATGTGGGAGAGGATGAGGAGGAGCTGGGCCAGCCGCTGGTGCTGCTGCTGCAGGGTCAGGCCTGCCTTGGCCATCAGGTGGATCAAAGTGTCTGTGATCTTGTCCAGGACTCGGTGGATATGGTCCTTCTCTTCCAGAGACTTCAGGGTGCTGGACAGAAATGTGTACACTCCAGAATTAAGCAAAATAATAGATTTGAGGCACACAAACTCCTCTCCCTGCAGATTCATCATGCGGAACCGAGATGATGTAGCCAGCAGCATGTCGAAGATCTCCACCATGCCCTCTACACATTTTCCCTGGTTCCTGTCCAAGAGCAAGTTAGGAGCAAACAGTAGCTTCACTGGGTGCTCCATGGAGCGCCAGACGAGACCAATCATCAGGATCTCTAGCCAGGCACATTCTAGAAGGTGGACCTGATCATGGAGGGTCAAATCCACAAAGCCTGGCACCCTCTTCGCCCAGTTGATCATGTGAACCAGCTCCCTGTCTGCCAGGTTGGTCAGTAAGCCCATCATCGAAGCTTCACTGAAGGGTCTGGTAGGATCATACTCGGAATAGAGTATGGGGGGCTCAGCATCCAACAAGGCACTGACCATCTGGTCGGCCGTCAGGGACAAGGCCAGGCTGTTCTTCTTAGAGCGTTTGATCATGAGCGGGCTTGGCCAAAGGTTGGCAGCTCTCATGTCTCCAGCAGATGGATCCTCTAGAGTCGACGCAGCTGCAGGGGTACCCAACAATGCTTTTATATCCTGTAAAGAATCCATTTTCAAAATCATGTCAAGGTCTTCTCGAGGAAAAATCAGTAGAAATAGCTGTTCCAGTCTTTCTAGCCTTGATTCCACTTCTGTCAGATGTGCCCTAGTCAGCGGAGACCTTTTGGTTTTGGGAGAGTAGCGACACTCCCAGTTGTTCTTCAGACACTTGGCGCACTTCGGTTTTTCTTTGGAGCACTTGAGCTTTTTAAGTCGGCAAATATCGCATGCTTGTTCGATAGAAGACAGTAGCTTCATCTTTCAGGAGGCTTGCTTCAAGCTTATCGATACCGTCGACCTCGAGGGGGGGCCCTGTTAATTCAGTAAATTTTCGATCTTGGGAAGAAAAAAGCAGTAAGCGTGAAAAATCTAAAAGCTGATGTAGTAGAAGATCCTATTCTTTAACAAAGATTGACCTTTTCTTTTTCTTCTTGGTTTGAGTAGAAAGGGGAAGGAAGAATACAAGAGAGAGGAAAAAAAGGAAGATAAAAAGAGAGCGTGATATAAATGAATATATATTAAACAAGAGAGATTGGGAAGGAAAGGATCAAACAAACCCAAAAATATTTCAAAAAGGAGAGAGAGAGGCGAGTTTGGTTTCAAAACGGTTTATTTATTTATGCAAGAGGACGTGGAAGAAAAAGAAGAAGGAAGAAAAAAATTTGAAAGAAAAAAACGCGTGGCGGGTAAAGAAGAAAATGGAAAATAGAGGCCGGGTGACAGAGAAATATTGAGGGTTAATTGGAAAATATGTTAGGGTGAGGCATATGTTTTTAAGGGTTTTGAGGATCCGATAAGGAAGAATGTAGGTTAAATGTTGTGCATTAATTGCTGTGGCAGCTTACCCGCTTCCCCACACATTTTGGTAGTATCTGTCCTCTTGTTATTGTTACTGTAATTGTGTATATATGTTCTCGCGTGTGTCTTATTTACTTATTTAGTTATTATATTATATGGGTCTGCAAGGTAGAGGCGTCGTGACTGGGAAAACCCTGGCGGACACTGGATGGCGGCGTTAGTATCGAATCGACAGCAGTATAGCGACCAGCATTCACATACGATTGACGCATGATATTACTTTCTGCGCACTTAACTTCGCATCTGGGCAGATGATGTCGAGGCGAAAAAAAATATAAATCACGCTAACATTTGATTAAAATAGAACAACTACAATATAAAAAAACTATACAAATGACAAGTTCTTGAAAACAAGAATCTTTTTATTGTCAGTACTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATATCTGGAATGCTGTTTTGCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTCGAAACGTGAGTCTTTTCCTTACCCATGGTTGTTTATGTTCGGATGTGATGTGAGAACTGTATCCTAGCAAGATTTTAAAAGGAAGTATATGAAAGAAGAACCTCAGTGGCAAATCCTAACCTTTTATATTTCTCTACAGGGGCGCGGCGTGGGGACAATTCAACGCGTCTGTGAGGGGAGCGTTTCCCTGCTCGCAGGTCTGCAGCGAGGAGCCGTAATTTTTGCTTCGCGCCGTGCGGCCATCAAAATGTATGGATGCAAATGATTATACATGGGGATGTATGGGCTAAATGTACGGGCGACAGTCACATCATGCCCCTGAGCTGCGCACGTCAAGACTGTCAAGGAGGGTATTCTGGGCCCCCATGTCGCTGGCCGGGTGACCCGGCGGGGACGAGGCAAGCTAAACAGATCTGGCGCGCCGGTGCACTCTCAGTACAATCTGCTCTGATGCTGGGGGAACAACTTCACAGAATGTTTTGTCATATTGTCGAAGTGGTCACAAAACAAGAGAAGTTCCGCCAATTATAAAAAGGGAACCCGTATATTTCAGCTTCACGGATGATTTCCAGGGTGAGAGTACTGTATATGGGCTTACGATAGAAGGCCATAAAAATTTCTTGCTTGGCAACAAAATAGAAGTGAAATCATGTCGAGGCTGCTGTGTGGGAGAACAGCTTAAAATATCACAAAAAAAGAATCTAAAACACTGTGTTGCTTGTCCCAGAAAGGGAATCAAGTATTTTTATAAAGATTGGAGTGGTAAAAATCGAGTATGTGCTAGATGCTATGGAAGATACAAATTCAGCGGTCATCACTGTATAAATTGCAAGTATGTACCAGAAGCACGTGAAGTGAAAAAGGCAAAAGACAAAGGCGAAAAATTGGGCATTACGCCCGAAGGTTTGCCAGTTAAAGGACCAGAGTGTATAAAATGTGGCGGAATCTTACAGTTTGATGCTGTCCGCGGGCCTCATAAGAGTTGTGGTAACAACGCAGGTGCGCGCATCTGCTAAGTTTAAACGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGA",
  "material": "dna",
  "sequence": "TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCGTTTAAACTTAGCAGATGCGCGCACCTGCGTTGTTACCACAACTCTTATGAGGCCCGCGGACAGCATCAAACTGTAAGATTCCGCCACATTTTATACACTCTGGTCCTTTAACTGGCAAACCTTCGGGCGTAATGCCCAATTTTTCGCCTTTGTCTTTTGCCTTTTTCACTTCACGTGCTTCTGGTACATACTTGCAATTTATACAGTGATGACCGCTGAATTTGTATCTTCCATAGCATCTAGCACATACTCGATTTTTACCACTCCAATCTTTATAAAAATACTTGATTCCCTTTCTGGGACAAGCAACACAGTGTTTTAGATTCTTTTTTTGTGATATTTTAAGCTGTTCTCCCACACAGCAGCCTCGACATGATTTCACTTCTATTTTGTTGCCAAGCAAGAAATTTTTATGGCCTTCTATCGTAAGCCCATATACAGTACTCTCACCCTGGAAATCATCCGTGAAGCTGAAATATACGGGTTCCCTTTTTATAATTGGCGGAACTTCTCTTGTTTTGTGACCACTTCGACAATATGACAAAACATTCTGTGAAGTTGTTCCCCCAGCATCAGAGCAGATTGTACTGAGAGTGCACCGGCGCGCCAGATCTGTTTAGCTTGCCTCGTCCCCGCCGGGTCACCCGGCCAGCGACATGGGGGCCCAGAATACCCTCCTTGACAGTCTTGACGTGCGCAGCTCAGGGGCATGATGTGACTGTCGCCCGTACATTTAGCCCATACATCCCCATGTATAATCATTTGCATCCATACATTTTGATGGCCGCACGGCGCGAAGCAAAAATTACGGCTCCTCGCTGCAGACCTGCGAGCAGGGAAACGCTCCCCTCACAGACGCGTTGAATTGTCCCCACGCCGCGCCCCTGTAGAGAAATATAAAAGGTTAGGATTTGCCACTGAGGTTCTTCTTTCATATACTTCCTTTTAAAATCTTGCTAGGATACAGTTCTCACATCACATCCGAACATAAACAACCATGGGTAAGGAAAAGACTCACGTTTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGCAAAACAGCATTCCAGATATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGCTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGTACTGACAATAAAAAGATTCTTGTTTTCAAGAACTTGTCATTTGTATAGTTTTTTTATATTGTAGTTGTTCTATTTTAATCAAATGTTAGCGTGATTTATATTTTTTTTCGCCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATATCATGCGTCAATCGTATGTGAATGCTGGTCGCTATACTGCTGTCGATTCGATACTAACGCCGCCATCCAGTGTCCGCCAGGGTTTTCCCAGTCACGACGCCTCTACCTTGCAGACCCATATAATATAATAACTAAATAAGTAAATAAGACACACGCGAGAACATATATACACAATTACAGTAACAATAACAAGAGGACAGATACTACCAAAATGTGTGGGGAAGCGGGTAAGCTGCCACAGCAATTAATGCACAACATTTAACCTACATTCTTCCTTATCGGATCCTCAAAACCCTTAAAAACATATGCCTCACCCTAACATATTTTCCAATTAACCCTCAATATTTCTCTGTCACCCGGCCTCTATTTTCCATTTTCTTCTTTACCCGCCACGCGTTTTTTTCTTTCAAATTTTTTTCTTCCTTCTTCTTTTTCTTCCACGTCCTCTTGCATAAATAAATAAACCGTTTTGAAACCAAACTCGCCTCTCTCTCTCCTTTTTGAAATATTTTTGGGTTTGTTTGATCCTTTCCTTCCCAATCTCTCTTGTTTAATATATATTCATTTATATCACGCTCTCTTTTTATCTTCCTTTTTTTCCTCTCTCTTGTATTCTTCCTTCCCCTTTCTACTCAAACCAAGAAGAAAAAGAAAAGGTCAATCTTTGTTAAAGAATAGGATCTTCTACTACATCAGCTTTTAGATTTTTCACGCTTACTGCTTTTTTCTTCCCAAGATCGAAAATTTACTGAATTAACAGGGCCCCCCCTCGAGGTCGACGGTATCGATAAGCTTGAAGCAAGCCTCCTGAAAGATGAAGCTACTGTCTTCTATCGAACAAGCATGCGATATTTGCCGACTTAAAAAGCTCAAGTGCTCCAAAGAAAAACCGAAGTGCGCCAAGTGTCTGAAGAACAACTGGGAGTGTCGCTACTCTCCCAAAACCAAAAGGTCTCCGCTGACTAGGGCACATCTGACAGAAGTGGAATCAAGGCTAGAAAGACTGGAACAGCTATTTCTACTGATTTTTCCTCGAGAAGACCTTGACATGATTTTGAAAATGGATTCTTTACAGGATATAAAAGCATTGTTGGGTACCCCTGCAGCTGCGTCGACTCTAGAGGATCCATCTGCTGGAGACATGAGAGCTGCCAACCTTTGGCCAAGCCCGCTCATGATCAAACGCTCTAAGAAGAACAGCCTGGCCTTGTCCCTGACGGCCGACCAGATGGTCAGTGCCTTGTTGGATGCTGAGCCCCCCATACTCTATTCCGAGTATGATCCTACCAGACCCTTCAGTGAAGCTTCGATGATGGGCTTACTGACCAACCTGGCAGACAGGGAGCTGGTTCACATGATCAACTGGGCGAAGAGGGTGCCAGGCTTTGTGGATTTGACCCTCCATGATCAGGTCCACCTTCTAGAATGTGCCTGGCTAGAGATCCTGATGATTGGTCTCGTCTGGCGCTCCATGGAGCACCCAGTGAAGCTACTGTTTGCTCCTAACTTGCTCTTGGACAGGAACCAGGGAAAATGTGTAGAGGGCATGGTGGAGATCTTCGACATGCTGCTGGCTACATCATCTCGGTTCCGCATGATGAATCTGCAGGGAGAGGAGTTTGTGTGCCTCAAATCTATTATTTTGCTTAATTCTGGAGTGTACACATTTCTGTCCAGCACCCTGAAGTCTCTGGAAGAGAAGGACCATATCCACCGAGTCCTGGACAAGATCACAGACACTTTGATCCACCTGATGGCCAAGGCAGGCCTGACCCTGCAGCAGCAGCACCAGCGGCTGGCCCAGCTCCTCCTCATCCTCTCCCACATCAGGCACATGAGTAACAAAGGCATGGAGCATCTGTACAGCATGAAGTGCAAGAACGTGGTGCCCCTCTATGACCTGCTGCTGGAGATGCTGGACGCCCACCGCCTACATGCGCCCACTAGCCGTGGAGGGGCATCCGTGGAGGAGACGGACCAAAGCCACTTGGCCACTGCGGGCTCTACTTCATCGGAGCTCCACTTAGACGGCGAGGACGTGGCGATGGCGCATGCCGACGCGCTAGACGATTTCGATCTGGACATGTTGGGGGACGGGGATTCCCCGGGTCCGGGATTTACCCCCCACGACTCCGCCCCCTACGGCGCTCTGGATATGGCCGACTTCGAGTTTGAGCAGATGTTTACCGATGCCCTTGGAATTGACGAGTACGGTGGGTAGGGATCCGGTTCCGGAAGTGGATCCGGATCCTGATACCGTCGACCTCGAGTCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGATGTCGTAATAACCCCGCCCCGACGCCATTTTAAGTCCAAAGGCACAATTTTACGTTGGAACTAAACGGTAAAGATCAAATATCACCTTTATTCTTTTTTGGTATGCATCGATTTCTTTGCCAGTAAGAACTACGTGCAACTCTACATTAGATCAAAATATAACGGATCATAAATGCAAACAACATTTGTCCAAAAGAAACAAAAATGTTGGCGTTTGTCTCGAACATGACACAATACTCCTTGATCGAGGGACATTGCCCGATTAACTATGATTTATACTTTTTACTACAGGTACTCGTTCTCCCTACTATTTCACCAGTATACGGTCGTTTTATGATTCATTTATTGCCTATAATGGTAAAAAGGGACATCTTAACCTTACGTGTTGCAATGAGACCATTCTTTTCGATTCATCAACTGTGAAATTTCTGAAATCCAGTACAATGCGAAACGCTACCGATAATGGCACCGTCTTTTGAATTATGAGGCCCAGATATGGCGTTCAATCTTCAACAAAAAAATGAATATCAAAACTAAATTCTATCTGGATAACAATCTTTCTTTGGTTTGCAGAAATATCATTAAAAAACAATCCATAACTACTAAGGAAGGGTATAAATGGTTTGGTTTACTAATTTTATATTTTGATTTGATTAGCAATTATAATCTCTAATATACTATTTCTGTAGCCAAAAATCTGCAATGTCAATAAGTAGCAAGTGCCGCTTACTCGTGAGAATATCAACCTTATAGCATATGTTTTCTATTTTTTTTTTTTGCTTATGGAGATAATGAACATTGTACACATGAACAAAGTGGTAGTTCTCTCTCCTTGATTTTTTGTGCTTGCGATATATATAATTGTTACATTCAATGGCCATGCTAAATAAAGGTTTTAGCTTTGTTTCACTCGTGCCCAGGATAATTGTTTAAACCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTC",
  "circular": true,
  "name": "pMODKan_HO_pACT1GE"
}