Electronic clicker for manual counting of three cell types (G1, G2/M and S). While looking down the eyepiece of a microscope at cells on a glass slide, the user can press "g" to increment the number of cells in G1, "h" to increment the number of cells in G2/M and "j" to increment the number of cells in S. Press space bar to move from one slide t…

CellCounter.py

import winsound, time, Tkinter

slides=[]
cells=[0,0,0]
labels=["G1","S","G2/M"]
print "Slide: %02d"%(len(slides)+1)

def key(event):
    if event.char=="g":
        cells[0]+=1
        winsound.Beep(220,100)
        print cells
    if event.char=="h":
        cells[1]+=1
        winsound.Beep(440,100)
        print cells
    if event.char=="j":
        cells[2]+=1
        winsound.Beep(880,100)
        print cells
    if event.char==" ":
        slides.append(cells[:])
        cells[:]=[0,0,0]
        print "Slide: %02d"%(len(slides)+1)
        winsound.Beep(330,100)
        winsound.Beep(660,100)
        winsound.Beep(1320,100)
        print cells
    if event.char=="q":
        root.destroy()

root = Tkinter.Tk()
frame = Tkinter.Frame(root, width=0, height=0)
frame.bind("<Key>", key)
frame.pack()
frame.focus_set()
root.mainloop()

slides.append(cells[:])

timestamp=time.strftime("%Y-%m-%d_%H-%M-%S", time.localtime())
f=open("CellReport_"+timestamp+".txt","w")
labels.insert(0,"Slide")
f.write("\t".join(labels)+"\n")

for s in xrange(0,len(slides)):
    slide=slides[s]
    slide.insert(0,s+1)
    f.write("\t".join(["%04d "%sl for sl in slide])+"\n")
f.close()