This document shows how to use a Ventoy formatted USB device (instead of an optical disk) to boot an ISO image in VirtualBox. There are other HowTo documents on the internet that explain this procedure (one is mentioned in the References section below), but most use a deprecated VBoxManage sub-command to create the raw device. This document uses the preferred createmedium sub-command. It also uses PowerShell rather than cmd.exe.
The generic form of the command that makes the Ventoy USB drive available to VirtualBox is as follows:
.\VBoxManage createmedium disk --filename=PATH --format=VMDK --variant=RawDisk --property RawDrive=DEVICE_NAME
But, there are some prerequisites...