parser.py

data = r"""File Name : File1.txt
Directory : Path\to\File1.txt
Created : 2012:04:15 10:15:32
Accessed : 2012:04:18 13:28:18"""
items = []
for line in data.split('\n'):
    type = line.split(':')[0].strip()
    rest = ':'.join(map(lambda x: x.strip(), line.split(':')[1:]))
    if type == 'File Name':
        items.append({type: rest})
    else:
        items[-1][type] = rest

print ','.join(items[0].iterkeys())

for dictionary in items:
    print ','.join(dictionary.itervalues())