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Build kb-python reference for GENCODE release M26 GRCm39
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## This is only on compute canada clusters where modules are available! | |
module load python/3.7.4 | |
## Create virtual environment using python | |
python3 -m venv ./kb_python_env | |
## activate environment | |
source ./kb_python_env/bin/activate | |
## Install kb-python inside environment | |
pip3 install kb-python | |
## Download references from Gencode | |
mkdir references | |
cd ./references | |
wget ftp://ftp.ebi.ac.uk/pub/databases/gencode/Gencode_mouse/release_M26/GRCm39.primary_assembly.genome.fa.gz | |
wget ftp://ftp.ebi.ac.uk/pub/databases/gencode/Gencode_mouse/release_M26/gencode.vM26.annotation.gtf.gz | |
cd .. | |
kb ref --workflow standard -i ./references/kb_ref.GRCm39.idx -g ./references/t2g_kb_ref.GRCm39.txt -f1 ./references/cdna.GRCm39 ./references/GRCm39.primary_assembly.genome.fa.gz ./references/gencode.vM26.annotation.gtf.g |
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