An example of protocols.io JSON output with steps included.

protocolsiojsonwithsteps.json

{
	"api_version": "1",
	"is_new_mode": "1",
	"protocol_name": "Transformation of Skeletonema marinoi using Multipulse Electroporation",
	"last_modified": "1487254340",
	"type_id": "1",
	"link": "",
	"fork_id": "",
	"public_fork_note": "",
	"number_of_steps": "7",
	"uri": "transformation-of-skeletonema-marinoi-using-multip-g6nbzde",
	"has_versions": "0",
	"first_published_date": "2017-02-10 10:06:56",
	"publish_date": "2017-02-10 10:06:56",
	"documents": null,
	"have_protocol_in_step": "0",
	"is_protocol_in_step": "0",
	"vendor_name": "Contributed by users",
	"vendor_link": "https://www.protocols.io",
	"vendor_logo": "/img/vendors/1.png",
	"mod_mins": "-34",
	"mod_secs": "34",
	"description": "<p><em>The following transformation protocol is designed for the insertion of linear DNA constructs into the nuclear genome of Skeletonema marinoi by non-homologous recombination.</em></p>",
	"is_bookmarked": "0",
	"can_reassign": "0",
	"before_start": "",
	"has_guidelines": "0",
	"materials": null,
	"warning": "",
	"is_prepublished": "0",
	"public": "1",
	"is_owner": "0",
	"is_original_owner": "0",
	"created_on": "1486720407",
	"authors": "Oskar N. Johansson., Adrian K. Clarke",
	"protocol_affiliation": "BioEnv - University of Gothenburg",
	"steps": [
		{
			"id": "319256",
			"is_changed": "0",
			"original_id": "0",
			"guid": "3A674EEDAB454907BEAB82DCDE053595",
			"previous_guid": null,
			"components": [
				{
					"component_type_id": "1",
					"name": "Description",
					"data": "<p>a) Grow two 400 mL cultures in Artificial Seawater with f/2+Si supplements (Growth media) for ca. one week until dense. The standard growth conditions used in our lab are 16°C, 50-70 µmol. photons m-2 s-1, 16 h photoperiod.</p>\n<p>b) Reduce the volume of the sedimented culture in each flask to 50 mL by suction. Resuspend cells by vigorous agitation to minimize chain length and then transfer them to a 50 mL centrifuge tube.</p>\n<p>c) Pellet cells by centrifugation (1200 x g, 5 min, 4°C, swing-out rotor). Decant supernatant and resuspend the pellet in each tube in 10 mL of ice-cold 0.3M sorbitol.</p>\n<p>d) Pellet cells once more by centrifugation (1200 x g, 5 min, 4°C, swing-out rotor) and resuspend in 2.5 mL of ice-cold 0.3M sorbitol. Pool both resuspensions in a single tube.</p>\n<p>e) Store the cell suspension on ice until needed. </p>",
					"data_id": "0"
				},
				{
					"component_type_id": "6",
					"name": "Section",
					"data": "Cell preparation",
					"data_id": "0"
				}
			]
		},
		{
			"id": "319300",
			"is_changed": "0",
			"original_id": "0",
			"guid": "0B729134C6634465829EEAD65EEDBA48",
			"previous_guid": "3A674EEDAB454907BEAB82DCDE053595",
			"components": [
				{
					"component_type_id": "1",
					"name": "Description",
					"data": "<p><em>This protocol was tested using the Gene Pulser Xcell electroporator (BioRad, USA) with the recommended 2 mm cuvettes (BioRad, USA).</em></p>",
					"data_id": "0"
				},
				{
					"component_type_id": "6",
					"name": "Section",
					"data": "ElectroporatEquipment",
					"data_id": "0"
				}
			]
		},
		{
			"id": "319306",
			"is_changed": "0",
			"original_id": "0",
			"guid": "4ED25CA215DA47A68682C9F63B759156",
			"previous_guid": "0B729134C6634465829EEAD65EEDBA48",
			"components": [
				{
					"component_type_id": "1",
					"name": "Description",
					"data": "<p>Add 3-5 μg of the linear DNA construct to a 100 μL cell suspension in a 2 mm electroporation cuvette and leave for 3-5 min at 4°C. <br /><br /><em>NB. DNA construct should include a selectable marker such as antibiotic resistance (e.g., zeocin/bleomycin)</em></p>",
					"data_id": "0"
				},
				{
					"component_type_id": "6",
					"name": "Section",
					"data": "Combining the cell suspension with desired DNA",
					"data_id": "0"
				}
			]
		},
		{
			"id": "319314",
			"is_changed": "0",
			"original_id": "0",
			"guid": "1EB656469EB44888A9119D250D34DEE2",
			"previous_guid": "4ED25CA215DA47A68682C9F63B759156",
			"components": [
				{
					"component_type_id": "1",
					"name": "Description",
					"data": "<p>Ensure cells are resuspended in the cuvette prior to electroporation. Electroporation is performed by the following steps:</p>\n<p> </p>\n<p>a) Poring pulses (300 V, 6 pulses, 1 sec pulse Interval, 5 ms pulse length)</p>\n<p>b) Transfer pulses (40 pulses [10x4], 50 ms pulse length, 0.1 s pulse interval)</p>\n<p>c) Reverse direction of the cuvette. </p>\n<p>d) Repeat transfer pulses (40 pulses [10x4], 50 ms pulse length, 0.1 s pulse interval)</p>\n<p> </p>",
					"data_id": "0"
				},
				{
					"component_type_id": "6",
					"name": "Section",
					"data": "Electroporation",
					"data_id": "0"
				}
			]
		},
		{
			"id": "319387",
			"is_changed": "0",
			"original_id": "0",
			"guid": "5DA068342E714C158A41C5433AC9EC64",
			"previous_guid": "1EB656469EB44888A9119D250D34DEE2",
			"components": [
				{
					"component_type_id": "1",
					"name": "Description",
					"data": "<p>Transfer the electroporated cells from the cuvette using a Pasteur pipette to 30 mL liquid growth media (without selection) and leave for 48 h under standard growth conditions</p>",
					"data_id": "0"
				},
				{
					"component_type_id": "6",
					"name": "Section",
					"data": "Cell recovery",
					"data_id": "0"
				}
			]
		},
		{
			"id": "319391",
			"is_changed": "0",
			"original_id": "0",
			"guid": "4D3CD4C8434C4143A4DEFCD344132F8A",
			"previous_guid": "5DA068342E714C158A41C5433AC9EC64",
			"components": [
				{
					"component_type_id": "1",
					"name": "Description",
					"data": "<p>Add selection to the media and continue growth for another 48 h</p>",
					"data_id": "0"
				},
				{
					"component_type_id": "6",
					"name": "Section",
					"data": "Apply Selection",
					"data_id": "0"
				}
			]
		},
		{
			"id": "319398",
			"is_changed": "0",
			"original_id": "0",
			"guid": "ECA2E8DAD3BC4EE698268C16F9FAB9C7",
			"previous_guid": "4D3CD4C8434C4143A4DEFCD344132F8A",
			"components": [
				{
					"component_type_id": "1",
					"name": "Description",
					"data": "<p>a) Pellet cells in 50 mL centrifuge tubes (1200 x g, 5 min, room temperature) and resuspend in 1 mL growth medium with selection.</p>\n<p>b) Spread 75 (for 7 cm diameter plates) or 250 µL (for 15 cm diameter plates) of the resuspension on growth media plates (f/2 + Si + selection, 0.9% Agar).</p>\n<p> </p>\n<p><em>Left under standard growth conditions, colonies should appear after 2-3 weeks. </em></p>",
					"data_id": "0"
				},
				{
					"component_type_id": "6",
					"name": "Section",
					"data": "Transfer to Solid media",
					"data_id": "0"
				}
			]
		}
	],
	"doi": "dx.doi.org/10.17504/protocols.io.g6nbzde",
	"doi_status": "2",
	"version_id": "0",
	"changed_fork_steps": null,
	"profile_url": "w2x234w2r2",
	"protocol_img": "https://s3.amazonaws.com/pr-journal/ia2dpr6.jpg",
	"profile_image": "/img/avatars/002.png",
	"full_name": "Adrian Clarke",
	"private_link": "87879142B7D5A76958A2335598E3456A",
	"original_img": "1",
	"username": "adrian-clarke",
	"is_retracted": "0",
	"retraction_reason": null,
	"first_name": "Adrian",
	"last_name": "Clarke",
	"affiliation": "",
	"created_by": "Adrian Clarke",
	"groups": [
		{
			"group_id": "108",
			"group_uri": "protist-research-to-optimize-tools-in-genetics-protg",
			"group_name": "Protist Research to Optimize Tools in Genetics (PROT-G)",
			"group_logo": "https://s3.amazonaws.com/pr-journal/e3addmn.png"
		}
	],
	"big_protocol_img": "https://s3.amazonaws.com/pr-journal/iazdpr6.jpg",
	"big_protocol_img_ofn": "skel.jpg",
	"number_of_comments": 4,
	"number_of_shared_runs": 0,
	"tags": [],
	"ownership_history": [
		{
			"change_time": "2017-02-10 11:52:22",
			"username": "oskar-johansson",
			"profile_image": "https://s3.amazonaws.com/pr-journal/icvdpr6.jpg",
			"full_name": "Oskar Johansson",
			"affiliation": "Biology and Environmental Sciences - Gothenburg University"
		}
	],
	"collections": [],
	"keywords": "",
	"contact_badges": [],
	"banner": null
}