Using cypress with react-dropzone to upload a file.

Dropzone.js

// your react component
import * as React from 'react'
import Dropzone from 'react-dropzone'

// apply your own styling and stuff, should probably also show the files when uploaded
const Upload = () => {
  return (<Dropzone data-cy="drag-and-drop">Drag and Drop here</Dropzone>)
}
          
export default Upload

index.spec.js

// cypress test
describe('Test', function() {
    it('Should be able upload', function() {
        // upload file using drag and drop using a fixtue
        cy.fixture('files/file.pdf', 'base64').then(content => {
            cy.get('[data-cy=drag-and-drop]').upload(content, 'filename.pdf')
        })

        // assert succesful upload and continue testing
    })
})

upload.js

// Custom comand to handle uploading a file using react-dropzone
// Should probably be placed somewhere in /support/

Cypress.Commands.add(
    'upload',
    {
        prevSubject: 'element',
    },
    (subject, file, fileName) => {
        // we need access window to create a file below
        cy.window().then(window => {
            // line below could maybe be refactored to make use of Cypress.Blob.base64StringToBlob, instead of this custom function.
            // inspired by @andygock, please refer to https://github.com/cypress-io/cypress/issues/170#issuecomment-389837191
            const blob = b64toBlob(file, '', 512)
            // Please note that we need to create a file using window.File,
            // cypress overwrites File and this is not compatible with our change handlers in React Code
            const testFile = new window.File([blob], fileName)
            cy.wrap(subject).trigger('drop', {
                dataTransfer: { files: [testFile] },
            })
        })
    }
)

// Code stolen from @nrutman here: https://github.com/cypress-io/cypress/issues/170
function b64toBlob(b64Data, contentType = '', sliceSize = 512) {
    const byteCharacters = atob(b64Data)
    const byteArrays = []

    for (let offset = 0; offset < byteCharacters.length; offset += sliceSize) {
        const slice = byteCharacters.slice(offset, offset + sliceSize)

        const byteNumbers = new Array(slice.length)
        for (let i = 0; i < slice.length; i++) {
            byteNumbers[i] = slice.charCodeAt(i)
        }

        const byteArray = new Uint8Array(byteNumbers)

        byteArrays.push(byteArray)
    }

    const blob = new Blob(byteArrays, { type: contentType })
    return blob
}

For anyone else running into this problem, I would like to share my solution which is an amalgamation of everything suggested here.

For an image e.g. image/jpeg, I used OP's @ZwaarContrast b64toBlob:

cy.fixture('image_file.jpg')
          .then(fileContent => {
            cy.get('input[type="file"]')
              .attachFile({
                fileContent: b64toBlob(fileContent),
                fileName: 'image_file.jpg',
                mimeType: 'image/jpeg',
              });
          });

For a video file e.g. video/mp4, I used Cypress's Cypress.Blob.binaryStringToBlob to get the blob:

        cy.fixture('video_file.mp4', 'binary')
          .then(Cypress.Blob.binaryStringToBlob)
          .then(blob => {
            cy.get('input[type="file"]').attachFile({
              fileContent: blob,
              fileName: 'video_file.mp4',
              mimeType: 'video/mp4',
              encoding: 'utf8',
            });
          });

For a audio file e.g. .mp3/audio/mpeg, I also used Cypress's Cypress.Blob.binaryStringToBlob:

        cy.fixture('audio_file.mp3', 'binary')
          .then(Cypress.Blob.binaryStringToBlob)
          .then(blob => {
            cy.get('input[type="file"]')
              .attachFile({
                fileContent: blob,
                fileName: 'audio_file.mp3',
                mimeType: 'audio/mpeg',
              });
          });

Lastly, for CSV files, the following worked via drag and drop:

cy.get('.dropzone').attachFile('csv_file.csv', { subjectType: 'drag-n-drop' })

I am using the following package versions:

    "cypress": "^11.2.0",
    "react": "17.0.2",
    "react-dropzone": "^11.3.4",