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FASTQC_VERSION=0.4.3 | |
SRCDIR=/usr/local/src/ | |
cd $SRCDIR && mkdir -p fastqc && cd fastqc | |
wget http://www.bioinformatics.bbsrc.ac.uk/projects/fastqc/fastqc_v${FASTQC_VERSION}.zip | |
unzip fastqc_v${FASTQC_VERSION}.zip | |
cd FastQC && chmod a+x fastqc |
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SRCDIR=/usr/local/src/ | |
wget http://research-pub.gene.com/gmap/src/gmap-gsnap-2010-07-20.tar.gz | |
tar xzvf gmap* | |
cd gmap* | |
./configure | |
make -j4 | |
sudo make install |
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SRCDIR=/usr/local/src/ | |
BOWTIE_VERSION=0.12.7 | |
TOPHAT_VERSION=1.0.13 | |
CUFFLINKS_VERSION=0.8.2 | |
FASTX_VERSION=0.0.13 | |
BEDTOOLS_VERSION=2.7.1 | |
cd $SRCDIR | |
# samtools | |
svn co https://samtools.svn.sourceforge.net/svnroot/samtools/trunk/samtools samtools-svn | |
cd samtools-svn && make | |
hg clone https://pysam.googlecode.com/hg/ pysam-hg | |
cd pysam-hg && python setup.py import ../ && sudo python setup.py install | |
cd $SRCDIR | |
# bowtie | |
mkdir -p bowtie && cd bowtie | |
wget http://downloads.sourceforge.net/project/bowtie-bio/bowtie/${BOWTIE_VERSION}/bowtie-${BOWTIE_VERSION}-src.zip?use_mirror=iweb | |
unzip bowtie-${BOWTIE_VERSION}-src.zip && cd bowtie-${BOWTIE_VERSION} && make | |
cd $SRCDIR | |
# tophat | |
mkdir -p tophat | |
cd tophat | |
wget http://tophat.cbcb.umd.edu/downloads/tophat-${TOPHAT_VERSION}.tar.gz | |
tar xzvf tophat-${TOPHAT_VERSION}.tar.gz | |
cd tophat-${TOPHAT_VERSION} && ./configure && make && sudo make install | |
# cufflinks ( requires libboost-dev, libboost-thread-dev ) | |
# may have to add explicit char * cast at: src/hits.cpp:394) | |
# and see this: http://stackoverflow.com/questions/1253245/installing-libboost-1-38-on-ubuntu-8-10/1254884#1254884 on installing boost from src (cufflinks requires boost > 1.38) | |
cd $SRCDIR | |
mkdir -p cufflinks | |
cd cufflinks | |
wget http://cufflinks.cbcb.umd.edu/downloads/cufflinks-${CUFFLINKS_VERSION}.tar.gz | |
tar xzvf cufflinks-${CUFFLINKS_VERSION}.tar.gz | |
cd cufflinks-${CUFFLINKS_VERSION} && ./configure && make && sudo make install | |
cd $SRCDIR | |
mkdir -p supersplat | |
cd supersplat | |
wget http://supersplat.cgrb.oregonstate.edu/files/supersplat_source.tgz | |
tar xzvf supersplat_source.tgz | |
make | |
cd $SRCDIR | |
mkdir -p fastx | |
cd fastx | |
wget http://hannonlab.cshl.edu/fastx_toolkit/libgtextutils-0.6.tar.bz2 | |
bunzip2 libgtextutils-0.6.tar.bz2 && tar xvf libgtextutils-0.6.tar | |
cd libgtextutils-0.6 && ./configure && make && sudo make install && sudo ldconfig | |
cd ../ | |
wget http://hannonlab.cshl.edu/fastx_toolkit/fastx_toolkit-${FASTX_VERSION}.tar.bz2 | |
bunzip2 fastx_toolkit-${FASTX_VERSION}.tar.bz2 | |
tar xvf fastx_toolkit-${FASTX_VERSION}.tar | |
cd fastx_toolkit-${FASTX_VERSION} && ./configure && make && sudo make install | |
cd $SRCDIR | |
# BED Tools | |
mkdir -p bedtools | |
cd bedtools | |
wget http://bedtools.googlecode.com/files/BEDTools.v${BEDTOOLS_VERSION}.tar.gz | |
tar xzvf BEDTools.v${BEDTOOLS_VERSION}.tar.gz | |
cd BEDTools && make | |
cd $SRCDIR | |
svn co https://htseq.svn.sourceforge.net/svnroot/htseq/trunk htseq-svn | |
cd htseq-svn | |
sudo python setup.py build install | |
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# progs for NGS | |
# this is required for command line tools | |
sudo apt-get install libbcel-java | |
svn co https://picard.svn.sourceforge.net/svnroot/picard/trunk picard-svn | |
cd picard-svn && svn up | |
env JAVA_HOME=/usr/lib/jvm/java-6-openjdk/ ant sam-jar | |
env JAVA_HOME=/usr/lib/jvm/java-6-openjdk/ ant -lib lib/ant package-commands | |
# docs: http://picard.sourceforge.net/command-line-overview.shtml |
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