Created
April 28, 2017 10:31
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motif finder
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#not /usr/local/bin/perl | |
# Reports a match and position of a Motif | |
# | |
# Input: | |
# One motif pattern file | |
# One sequence file | |
$patternFile; | |
$searchFile; | |
displayPrompt(); | |
printf "\nMotif pattern file: " . $patternFile . "\n" . "Sequence file: " . $patternFile . "\n"; | |
$patternText = readFile($patternFile); | |
$searchText = readFile($searchFile); | |
print "\nMotif pattern text:\n" . $patternText . "\nSequence text:\n" . $searchText . "\n"; | |
$start = 0; | |
$end = length($searchText) - length($patternText); | |
for($start; $start < $end; $start++) | |
{ | |
$matchSequence = substr($searchText, $start, length($patternText)); | |
if($patternText eq $matchSequence) | |
{ | |
print "\nMOTIF FOUND AT POSITION " . $start; | |
} | |
} | |
#print $searchText =~ /$patternText/; | |
sub displayPrompt | |
{ | |
while(not defined($patternFile)) | |
{ | |
$testFile = getInput("Please enter a motif pattern file.\n\t"); | |
if(-f $testFile) | |
{ | |
$patternFile = $testFile; | |
} | |
else | |
{ | |
printf "\n\"$testFile\" does not exist in the current directory.\n" | |
} | |
} | |
while(not defined($searchFile)) | |
{ | |
$testFile = getInput("Please enter a sequence file.\n\t"); | |
if(-f $testFile) | |
{ | |
$searchFile = $testFile; | |
} | |
else | |
{ | |
printf "\n\"$testFile\" does not exist in the current directory.\n" | |
} | |
} | |
} | |
sub getInput | |
{ | |
my $prompt = shift; | |
$prompt = "" if not defined $prompt; | |
$files = ""; | |
if(defined($patternFile)) | |
{ | |
$files = "\Motif pattern file: " . $patternFile . "\n"; | |
} | |
else | |
{ | |
$files = "\Motif pattern file: NONE\n"; | |
} | |
if(defined($searchFile)) | |
{ | |
$files = $files . "Sequence file: " . $patternFile . "\n"; | |
} | |
else | |
{ | |
$files = $files . "Sequence file: NONE\n"; | |
} | |
$prompt = $files . $prompt; | |
print($prompt); | |
$input = <>; | |
chomp($input); | |
return $input; | |
} | |
sub readFile | |
{ | |
my $file = shift; | |
print "\nRead from file " . $file; | |
if(not -f $file) | |
{ | |
printf "\n$file is not a valid file in the current directory.\n"; | |
exit; | |
} | |
$text = ""; | |
if (open(my $fh, '<:encoding(UTF-8)', $file)) | |
{ | |
while(my $line = <$fh>) | |
{ | |
if(not eof) | |
{ | |
chomp $line; | |
$text = $text . uc($line); # Uppercase | |
} | |
} | |
} | |
else | |
{ | |
print "\nError reading file: " . $file; | |
} | |
return $text; | |
} | |
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