Perl script that finds cases where a transcript listed in the 'genes' table of the TreeFam mysql database lacks any amino acid sequence in the 'aa_seq' table, or lacks a DNA sequence in the 'nt_seq' table.

treefam_QC8.pl

#!/usr/local/bin/perl

# 
# Perl script treefam_QC8.pl
# Written by Avril Coghlan ([email protected])
# 4-Feb-09.
#
# This perl script finds cases where a transript listed in the 'genes' table
# is missing an amino acid sequence in the 'aa_seq' table or missing a
# DNA sequence in the 'nt_seq' table.
#
# The command-line format is:
# % perl <treefam_QC8.pl> <release> 
#   where <release> is the release of the TreeFam database to use.
#
#
#------------------------------------------------------------------#

# CHECK IF THERE ARE THE CORRECT NUMBER OF COMMAND-LINE ARGUMENTS:

$num_args                  = $#ARGV + 1;
if ($num_args != 1)
{
   print "Usage of treefam_QC8.pl\n\n";
   print "perl treefam_QC8.pl <release>\n";
   print "where <release> is the release of the TreeFam database to use.\n";
   print "For example, >perl -w treefam_QC8.pl 7\n";
   exit;
}

#------------------------------------------------------------------#

# DECLARE MYSQL USERNAME AND HOST:
use DBI;

# FIND WHICH RELEASE OF THE TREEFAM DATABASE TO USE:

$release                   = $ARGV[0];

$VERBOSE                   = 1;

#------------------------------------------------------------------#

$database                  = "dbi:mysql:treefam_".$release.":db.treefam.org:3308";
$dbh                       = DBI->connect("$database", 'anonymous', '') || return;

# READ IN ALL THE TRANSCRIPTS THAT HAVE AMINO ACID SEQUENCES IN THE
# aa_seq TABLE:

$AASEQ                     = &read_aaseq($dbh);

# READ IN ALL THE TRANSCRIPTS THAT HAVE DNA SEQUENCES IN THE
# nt_seq TABLE:

$DNASEQ                    = &read_dnaseq($dbh);

# CHECK THAT ALL OF THE GENES IN THE genes TABLE HAVE DNA AND AMINO
# ACID SEQUENCES:

&read_genes($dbh,$AASEQ,$DNASEQ);

# NOW DISCONNECT FROM THE DATABASE:

$rc                        = $dbh->disconnect();
$rc                        = "";

#------------------------------------------------------------------#

print STDERR "FINISHED.\n";

#------------------------------------------------------------------#

# READ IN ALL THE TRANSCRIPTS THAT HAVE DNA SEQUENCES IN THE
# nt_seq TABLE:

sub read_dnaseq
{
   my $dbh                 = $_[0];
   my $table_w;
   my $st;
   my $sth;
   my $rv;
   my @array;
   my $ID;
   my %DNASEQ              = ();

   $table_w                = 'nt_seq';
   $st                     = "SELECT ID from $table_w";
   $sth                    = $dbh->prepare($st) or die "Cannot prepare $st: $dbh->errstr\n";
   $rv                     = $sth->execute or die "Cannot execute the query: $sth->errstr";
   if ($rv >= 1)
   {
      while ((@array) = $sth->fetchrow_array)
      {
         $ID               = $array[0];
         $DNASEQ{$ID}      = 1;
      }
   }

   print STDERR "Read in transcripts with DNA sequences\n";
   print "Read in transcripts with DNA sequences\n";

   return(\%DNASEQ);
}

#------------------------------------------------------------------#

# READ IN ALL THE TRANSCRIPTS THAT HAVE AMINO ACID SEQUENCES IN THE
# aa_seq TABLE:

sub read_aaseq
{
   my $dbh                 = $_[0];
   my $table_w;
   my $st;
   my $sth;
   my $rv;
   my @array;
   my $ID;
   my %AASEQ               = ();

   $table_w                = 'aa_seq';
   $st                     = "SELECT ID from $table_w";
   $sth                    = $dbh->prepare($st) or die "Cannot prepare $st: $dbh->errstr\n";
   $rv                     = $sth->execute or die "Cannot execute the query: $sth->errstr";
   if ($rv >= 1)
   {
      while ((@array) = $sth->fetchrow_array)
      {
         $ID               = $array[0];
         $AASEQ{$ID}       = 1;
      }
   }

   print STDERR "Read in transcripts with amino acid sequences\n";
   print "Read in transcripts with amino acid sequences\n";

   return(\%AASEQ);
}

#------------------------------------------------------------------#

# CHECK THAT ALL OF THE TRANSCRIPTS IN THE genes TABLE HAVE DNA AND
# AMINO ACID SEQUENCES:

sub read_genes
{
   my $dbh                 = $_[0];
   my $AASEQ               = $_[1];
   my $DNASEQ              = $_[2];
   my $table_w;
   my $st;
   my $sth;
   my $rv;
   my @array;
   my $ID;

   $table_w                = 'genes';
   $st                     = "SELECT ID from $table_w";
   $sth                    = $dbh->prepare($st) or die "Cannot prepare $st: $dbh->errstr\n";
   $rv                     = $sth->execute or die "Cannot execute the query: $sth->errstr";
   if ($rv >= 1)
   {
      while ((@array) = $sth->fetchrow_array)
      {
         $ID               = $array[0];
         if (!($AASEQ->{$ID}))
         {
            print "WARNING: ID $ID appears in the genes table, but has no amino acid sequence in the aa_seq table\n";
         }
         if (!($DNASEQ->{$ID}))
         {
            print "WARNING: ID $ID appears in the genes table, but has no DNA sequence in the nt_seq table\n";
         }
      }
   }
}

#------------------------------------------------------------------#