Perl script that, given an input gff file, identifies exons that have a 0 bp intron between them, or are overlapping exons, and merges them.

merge_overlapping_exons.pl

#!/usr/bin/env perl

=head1 NAME

    merge_overlapping_exons.pl

=head1 SYNOPSIS
 
    merge_overlapping_exons.pl input_gff output_gff outputdir input_fasta
        where input_gff is the input gff file,
              output_gff is the output gff file,
              outputdir is the directory to write output files in,
              input_fasta is the input fasta file for the assembly.

=head1 DESCRIPTION

    This script takes an input gff file, and identifies exons that have a 0 bp
    intron between them (ie. are adjacent exons) or are overlapping exons, and merges
    them. The corrected exons are written to an output gff file.

=head1 VERSION
  
    Perl script last edited 22-Jul-2013.

=head1 CONTACT

    [email protected] (Avril Coghlan)

=cut

# 
# Perl script merge_overlapping_exons.pl
# Written by Avril Coghlan ([email protected])
# 22-Jul-13.
# Last edited 22-Jul-2013.
# SCRIPT SYNOPSIS: merge_overlapping_exons.pl: given an input gff file, identifies exons that have a 0 bp intron between them, or are overlapping exons, and merges them.
#
#------------------------------------------------------------------#

# CHECK IF THERE ARE THE CORRECT NUMBER OF COMMAND-LINE ARGUMENTS:

use strict;
use warnings;

# xxx
BEGIN { 
    unshift (@INC, '/nfs/users/nfs_a/alc/Documents/git/helminth_scripts/modules'); 
}

use HelminthGenomeAnalysis::AvrilGffUtils;
use HelminthGenomeAnalysis::AvrilFileUtils; 
use HelminthGenomeAnalysis::AvrilFastaUtils;

my $num_args               = $#ARGV + 1;
if ($num_args != 4)
{
    print "Usage of merge_overlapping_exons.pl\n\n";
    print "perl merge_overlapping_exons.pl <input_gff> <output_gff> <outputdir> <input_fasta_file>\n";
    print "where <input_gff> is the input gff file,\n";
    print "      <output_gff> is the output gff file,\n";
    print "      <outputdir> is the directory to write output files in,\n";
    print "      <input_fasta_file> is the input fasta file for the assembly\n";
    print "For example, >perl merge_overlapping_exons.pl final.gff final2.gff . TRIC.v1.fa\n";
    exit;
}

# FIND THE PATH TO THE INPUT GFF FILE:                     

my $input_gff              = $ARGV[0];

# FIND THE NAME OF THE OUTPUT GFF FILE:

my $output_gff             = $ARGV[1];

# FIND THE NAME OF THE DIRECTORY TO WRITE OUTPUT FILES IN:

my $outputdir              = $ARGV[2];

# FIND THE NAME OF THE INPUT FASTA FILE FOR THE ASSEMBLY:

my $input_fasta            = $ARGV[3];

#------------------------------------------------------------------#

# RUN THE MAIN PART OF THE CODE:

&run_main_program($input_gff,$output_gff,$outputdir,$input_fasta);

print STDERR "FINISHED.\n";

#------------------------------------------------------------------#

# RUN THE MAIN PART OF THE CODE:

sub run_main_program
{
   my $input_gff           = $_[0]; # THE INPUT GFF FILE
   my $output_gff          = $_[1]; # THE OUTPUT GFF FILE
   my $outputdir           = $_[2]; # THE DIRECTORY TO WRITE OUTPUT FILES IN
   my $input_fasta         = $_[3]; # THE INPUT ASSEMBLY FASTA FILE 
   my $input_fasta_obj     = $_[4]; # OBJECT FOR THE INPUT FASTA FILE
   my $contigs2len;                 # HASH TABLE OF THE LENGTHS OF SCAFFOLD/CONTIG SEQUENCES
   my $contigslen_file;             # FILE WITH THE LENGTHS OF SCAFFOLD/CONTIG SEQUENCES 
   my $contig;                      # A CONTIG/SCAFFOLD NAME 
   my $cds_gff;                     # GFF FILE OF 'CDS' FEATURES  
   my $feature_types;               # FEATURE TYPES TO PUT IN $cds_gff 
   my $returnvalue;                 # RETURN VALUE FROM A FUNCTION
   my $sorted_cds_gff;              # A SORTED VERSION OF $cds_gff 
   my $exon_gff;                    # GFF FILE OF 'exon' FEATURES
   my $sorted_exon_gff;             # A SORTED VERSION OF $exon_gff 
   my $contiglen;                   # LENGTH OF A SCAFFOLD 

   # GET THE LENGTHS OF THE SEQUENCES IN THE INPUT FASTA FILE, AND WRITE THEM OUT IN A TEMPORARY FILE:
   $input_fasta_obj        = HelminthGenomeAnalysis::AvrilFastaUtils->new(fasta_file => $input_fasta); 
   $contigs2len            = HelminthGenomeAnalysis::AvrilFastaUtils::build_contigs2len($input_fasta_obj);
   $contigslen_file        = HelminthGenomeAnalysis::AvrilFileUtils::make_filename($outputdir); # MAKE A TEMPORARY FILE IN THE CURRENT DIRECTORY
   open(CONTIGSLENFILE,">$contigslen_file") || die "ERROR: run_main_program: cannot open $contigslen_file\n";
   foreach my $contig (keys %{$contigs2len})
   {
      $contiglen           = $contigs2len->{$contig};
      # ADD ONE TO THE SCAFFOLD LENGTH, SO THAT add_flanking_region_to_gff_features FUNCTION WILL WORK, EVEN IF THE END OF AN EXON/CDS FEATURE
      # IS THE LAST BASE OF THE SCAFFOLD: 
      $contiglen           = $contiglen + 1;
      print CONTIGSLENFILE "$contig\t$contiglen\n";
   } 
   close(CONTIGSLENFILE);

   # GET A GFF FILE OF 'CDS' FEATURES:
   $cds_gff                = HelminthGenomeAnalysis::AvrilFileUtils::make_filename($outputdir); # MAKE A TEMPORARY FILE IN THE CURRENT DIRECTORY
   $feature_types          = ['CDS'];
   $returnvalue            = HelminthGenomeAnalysis::AvrilGffUtils::get_gff_lines_for_feature_types($input_gff,$feature_types,$cds_gff); 
   # SORT $cds_gff:
   $sorted_cds_gff         = HelminthGenomeAnalysis::AvrilFileUtils::make_filename($outputdir); 
   $returnvalue            = HelminthGenomeAnalysis::AvrilGffUtils::sort_gff($cds_gff,$sorted_cds_gff,'CDS');

   # GET A GFF FILE OF 'exon' FEATURES:
   $exon_gff               = HelminthGenomeAnalysis::AvrilFileUtils::make_filename($outputdir); # MAKE A TEMPORARY FILE IN THE CURRENT DIRECTORY
   $feature_types          = ['exon'];
   $returnvalue            = HelminthGenomeAnalysis::AvrilGffUtils::get_gff_lines_for_feature_types($input_gff,$feature_types,$exon_gff); 
   # SORT $exon_gff:
   $sorted_exon_gff        = HelminthGenomeAnalysis::AvrilFileUtils::make_filename($outputdir); 
   $returnvalue            = HelminthGenomeAnalysis::AvrilGffUtils::sort_gff($exon_gff,$sorted_exon_gff,'exon');
  
   # MERGE OVERLAPPING CDS FEATURES, OR FEATURES THAT HAVE A 0 bp INTRON BETWEEN THEM:
   $returnvalue            = HelminthGenomeAnalysis::AvrilGffUtils::merge_overlapping_cds($sorted_cds_gff,$sorted_exon_gff,$input_gff,$output_gff,$outputdir,$contigslen_file);

   # DELETE TEMPORARY FILES:
   system "rm -f $exon_gff";
   system "rm -f $cds_gff";
   system "rm -f $sorted_cds_gff";
   system "rm -f $sorted_exon_gff";
   system "rm -f $contigslen_file";
}

#------------------------------------------------------------------#