Created
September 24, 2015 18:46
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This code uses Autoprotocol-Python to generate Autoprotocol JSON for a simple bacterial incubation and OD600 measurement.
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| from autoprotocol.protocol import Protocol | |
| p = Protocol() | |
| # Set up the containers being referenced | |
| ctrl_bacteria = p.ref("DH5a", id="3209xifd029", cont_type="micro-1.5", discard=True) | |
| growth_plate = p.ref("growth_plate", id="3208kfhf394", cont_type="96-flat", storage="cold_4") | |
| # Dispense, transfer, cover the plate, incubate, measure OD600 | |
| p.dispense(growth_plate, "lb-broth-noAB", [{"column": "0", "volume": "150:microliter"}]) | |
| p.transfer(ctrl_bacteria.well(0), growth_plate.wells_from(0,4, columnwise=True), "2:microliter") | |
| p.cover(growth_plate, lid="low_evaporation") | |
| p.incubate(growth_plate, "warm_37", "12:hour", shaking=True) | |
| p.absorbance(growth_plate, growth_plate.wells_from(0,8, columnwise=True), "600:nanometer", "OD600") |
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