The MIT License (MIT)
Copyright (c) 2015 Ben Miles
Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions:
| from autoprotocol.protocol import Protocol | |
| p = Protocol() | |
| # Set up the containers being referenced | |
| ctrl_bacteria = p.ref("DH5a", id="3209xifd029", cont_type="micro-1.5", discard=True) | |
| growth_plate = p.ref("growth_plate", id="3208kfhf394", cont_type="96-flat", storage="cold_4") | |
| # Dispense, transfer, cover the plate, incubate, measure OD600 | |
| p.dispense(growth_plate, "lb-broth-noAB", [{"column": "0", "volume": "150:microliter"}]) | |
| p.transfer(ctrl_bacteria.well(0), growth_plate.wells_from(0,4, columnwise=True), "2:microliter") |
| { | |
| "refs": { | |
| "growth_plate": { | |
| "id": "3208kfhf394", | |
| "store": { | |
| "where": "cold_4" | |
| } | |
| }, | |
| "DH5a": { | |
| "discard": true, |
| import requests | |
| import json | |
| # Use autoprotocol-python package to generate autoprotocol | |
| import autoprotocol as ap | |
| # Example protocol | |
| p = ap.Protocol() | |
| con = p.ref("name", id=None, cont_type="96-flat", storage=None, discard=True) | |
| p.cover(con) |
| from autoprotocol_utilities.magnetic_helpers import get_mag_amplicenter, get_mag_frequency | |
| source_plate = protocol.ref( | |
| block_name, | |
| cont_type="96-deep-kf", | |
| storage="cold_80") | |
| protocol.dispense_full_plate( | |
| source_plate, | |
| "water", | |
| "900:microliter") |
