Setup an Amazon Instance (16 CPU, 122 GB RAM, 320 GB SDD) with Ubuntu 14.04 and NCBI BLAST+ from Bio-Linux 8

aws-blast-msc.sh

# Get Amazon AWS Command Line tool
brew install awscli

# Setup instance
aws configure # key, secret, eu-west-1, table
aws ec2 create-key-pair --key-name MyKeyPair --query 'KeyMaterial' --output text > MyKeyPair.pem
aws ec2 run-instances --image-id ami-f0b11187 --instance-type r3.4xlarge --region eu-west-1 --key-name MyKeyPair
aws ec2 describe-instances
aws ec2 authorize-security-group-ingress --group-id sg-86bfxxxx --protocol tcp --port 22 --cidr 0.0.0.0/0

# Setup users and password ssh login
ssh -i MyKeyPair.pem [email protected]
sudo passwd ubuntu # password for admin
sudo adduser mscstudent # password, MSc Student
sudo sed -ie 's/PasswordAuthentication no/PasswordAuthentication yes/' /etc/ssh/sshd_config
sudo service ssh restart

# Install software
sudo apt-get install htop git -y
sudo add-apt-repository ppa:nebc/bio-linux
sudo apt-get update
sudo apt-get install ncbi-blast+ -y