Title: Integrative 'Omics' Analysis of ME/CFS: Plasma Metabolomics, Single-Cell Transcriptomics, and Microbiome
Prompt:
Patients are being diagnosed with Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS). Please gather all available key findings on this condition, with a particular focus on 'omics' research. Specifically, concentrate on these areas:
- Plasma Metabolomics and Redox Imbalance: Research studies investigating the metabolic profiles and redox status in the plasma of ME/CFS patients. Identify key biomarkers and metabolic pathways implicated in the disease.
- Single-Cell Transcriptomics of the Immune System: Gather information on studies using single-cell transcriptomics to analyze the immune system in ME/CFS. Focus on identifying changes in gene expression, immune cell populations, and immune signaling pathways.
- Microbiome: Explore research into gut microbiome, bacteria genetics and its relation with ME/CFS.
Additionally, within these areas, I'm interested in:
- Key Biomarkers: For dysbiosis, metabolic impacts, and any other relevant areas.
- Detailed information from selected omics studies.
Pay particular attention to any identified biomarkers, experimental methodologies, and significant results.
Furthermore, determine:
- Integration of the selected studies.
- How studies are connected.
- Redundancies and conflicts.
Consider the relevance and potential integration of studies related to butyrate and the microbiome (like the Guo et al. 2023 paper).
This prompt is my best interpretation of Derya Unutmaz, MD's X post: https://x.com/DeryaTR_/status/1898486904208711848
Latest update: check out how 3 insane ways how I use Manus AI: https://x.com/cedric_chee/status/1898712345779765351