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@clintval
Last active December 23, 2019 05:07
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INFO Fields

Key Definition
SAMPLE Sample name
TYPE Variant Type: SNV Insertion Deletion Complex
DP Total depth
End Chromosome end position
VD Variant depth
AF Allele frequency
BIAS Strand bias info
REFBIAS Reference depth by strand
VARBIAS Variant depth by strand
PMEAN Mean position in reads
PSTD Standard deviation of position in reads
QUAL Mean quality score of reads
QSTD Standard deviation of quality score of reads
SBF Strand bias Fisher p-value
ODDRATIO Strand bias odd ratio
MQ Mean mapping quality
SN Signal to noise
HIAF Allele frequency using only high quality bases
ADJAF Adjusted allele frequency for indels due to local realignment
SHIFT3 Number of bases to be shifted to 3 prime for deletions due to alternative alignment
MSI Microsatellite - greater than 1 indicates MSI
MSILEN Microsatellite unit length in bp
NM Mean mismatches in reads
LSEQ 5' flanking sequence
RSEQ 3' flanking sequence
GDAMP Number of amplicons supporting variant
TLAMP Total of amplicons covering variant
NCAMP Number of amplicons don't work (WTF?)
AMPFLAG Top variant in amplicons don't match
HICNT High quality variant reads
HICOV High quality total reads

FILTER Fields

Key Definition
Q25 Mean base quality below 25
Q10 Mean mapping quality below 10
p8 Mean position in reads less than 8
SN1.5 Signal to noise ratio less than 1.5
Bias Strand bias
pSTD Position in reads has a standard deviation of 0
d3 Total depth < 3
v2 Variant depth < 2
f0.0 Allele frequency < 0.0
MSI12 Variant in MSI region with 12 non-monomer or 13 monomer MSI
NM4.25 Mean mismatches in reads >= 4.25 thus likely false positives
InGap The variant is in the deletion gap, thus likely false positive
InIns The variant is adjacent to an insertion variant
Cluster0bp Two variants are within 0 bp
LongMSI The somatic variant is flanked by a long A/T (>=14)
AMPBIAS Indicates the variant has amplicon bias

FORMAT Fields

Key Definition
GT Genotype
DP Total depth
VD Variant depth
AD Allelic depths for the REF and ALT alleles in the order listed
AF Allele frequency
RD Reference forward, reverse reads
ALD Variant forward, reverse reads
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