Created
September 2, 2021 00:16
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import com.fulcrumgenomics.bam.api.SamSource | |
import com.fulcrumgenomics.commons.CommonsDef._ | |
/** Namespace for SAM/BAM source utilities. */ | |
object SamSourceUtil { | |
/** Return the only sample in this SAM/BAM source otherwise raise an exception. */ | |
def onlySample(source: SamSource): String = { | |
validateHasSingleSample(source) | |
source.header.getReadGroups.map(_.getSample).toSeq.head | |
} | |
/** Return the only sample in this SAM/BAM file otherwise raise an exception. */ | |
def onlySample(bam: PathToBam): String = { | |
val source = SamSource(bam) | |
yieldAndThen(onlySample(source))(source.safelyClose()) | |
} | |
/** The sample names from the input SAM/BAM source. */ | |
def samples(source: SamSource): Seq[String] = { | |
source.header.getReadGroups.map(_.getSample).distinct.toSeq | |
} | |
/** The sample names from the input SAM/BAM file. */ | |
def samples(sam: PathToBam): Seq[String] = { | |
val source = SamSource(sam) | |
yieldAndThen(samples(source))(source.safelyClose()) | |
} | |
/** Validate that a SAM/BAM source has a single sample's reads. */ | |
def validateHasSingleSample(source: SamSource): Unit = { | |
val names = samples(source) | |
val n = names.length | |
if (n != 1) { | |
source.safelyClose() | |
throw new IllegalArgumentException(s"SAM/BAM is not single-sample and has $n samples: ${names.mkString(", ")}") | |
} | |
} | |
/** Validate that a SAM/BAM file has a single sample's reads. */ | |
def validateHasSingleSample(sam: PathToBam): Unit = { | |
val source = SamSource(sam) | |
yieldAndThen(validateHasSingleSample(source))(source.safelyClose()) | |
} | |
} |
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