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quality score distribution from fastq
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#!/usr/bin/env python | |
usage=""" | |
## quality score distribution from fastq | |
gunzip -c fastq.gz | python qualDist.py | |
""" | |
import sys | |
num = 0 | |
cnt = {} | |
all_q = [] | |
for line in sys.stdin: | |
num += 1 | |
if num%4 == 0: | |
for q in line.strip(): | |
# translate to ascii numeric value | |
q1 = ord(q) | |
#print "q1: %s" % q1 | |
all_q.append(q1) | |
# tally num occurences of each obs value | |
if q1 not in cnt: cnt[q1] = 0 | |
cnt[q1] += 1 | |
#print "cnt: %s" % cnt | |
print(len(all_q)) | |
avg_q = sum(all_q)/float(len(all_q)) | |
print "avg qual score: %s" % avg_q | |
## output distribution of values | |
#for k in cnt: | |
# print "score: %s count: %d" % (k, cnt[k]) | |
# print k, cnt[k] |
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