if you have mkisofs/genisoimage and say your folder is named "ISOTMP"
genisoimage -r -V "ISO-LABEL" -cache-inodes -J -l \
-b isolinux/isolinux.bin -c isolinux/boot.cat -no-emul-boot \
-boot-load-size 4 -boot-info-table -o NAME-OF-ISO.iso ISOTMP/
mkisofs -r -V "ISO-LABEL" -cache-inodes -J -l \
-b isolinux/isolinux.bin -c isolinux/boot.cat -no-emul-boot \
-boot-load-size 4 -boot-info-table -o NAME-OF-ISO.iso ISOTMP/