tk-grid-demo.py

#!/usr/bin/env python2
import Tkinter as tk

f = tk.Frame()
f.pack()

w1 = tk.Label(f, text='widget1')
w1.grid(column=0, row=0)
w2 = tk.Label(f, text='widget2')
w2.grid(column=120, row=12)

def debug_del(msg):
    print('-' * 16 + msg)
    for w in f.grid_slaves(column=4, row=13):
        print(w.cget('text'))

def delete():
    for w in f.grid_slaves(column=4, row=13):
        w.destroy()
        break
        # w.grid_remove()
    debug_del('delete')

def add():
    n = len(list(f.grid_slaves(column=4, row=13)))
    w4 = tk.Button(f, text='Delete ' + str(n), command=delete)
    w4.grid(column=4, row=13)
    debug_del('add')

w3 = tk.Button(f, text='Add', command=add)
w3.grid(column=3, row=14)

f.mainloop()