https://qiita.com/MachiaWorx/items/87968666a2baf9fcc493

sample_source.py

import math
import array
import io
import os
import os.path as path
import wave
import winsound

wav_hz = 44100
musictime = wav_hz * 5
frq = 440.0

def main():
    wave_data = array.array('h')
    for t in range(musictime):
        u = 12767 * math.sin(2.0 * math.pi * frq * t / wav_hz)
        wave_data.append(int(u))

    with io.BytesIO() as file:
        with wave.open(file, 'wb') as wav_file:
            wav_file.setnchannels(1)
            wav_file.setsampwidth(2)
            wav_file.setframerate(wav_hz)
            wav_file.writeframes(wave_data)
        winsound.PlaySound(file.getbuffer(), winsound.SND_MEMORY)


if __name__ == '__main__':
    main()