Long read RNA-seq costs

nanopore.md

Item	Cost	Number of experiments
Pack 4xPromethION Flow Cell	$4000	4-40
(multiplexing) cDNA-PCR Barcoding Kit V14	$750	144
(direct RNA) Direct RNA Sequencing Kit	$600	6
Induro® Reverse Transcriptase and 5x Induro® RT Reaction Buffer (NEB, M0681)	$200	20
RNAse inhibitor	$600	100
dNTP mix	$300	600
NEBNext® Quick Ligation Module	$400	20
RNAClean XP RNA and cDNA Cleanup Reagent, 40 mL	$1200	400
Qubit™ RNA High Sensitivity (HS)	$500	500
Qubit™ Assay Tubes	$100	500
High Sensitivity RNA ScreenTape Analysis	$400	100
---------	--------	--------
Total per xp	$150 (cDNA with multiplexing) - $1160 (direct RNA)	1

pacbio.md

Item	Cost	Number of experiments
Revio SPRQ sequencing plate	$4000	4-40
Kinnex full-length RNA kit	$700	12
Iso-Seq express 2.0 kit	$2400	24
Qubit™ RNA High Sensitivity (HS)	$500	500
Qubit™ Assay Tubes	$100	500
High Sensitivity RNA ScreenTape Analysis	$400	100
---------	--------	--------
Total per xp	$270 (with multiplexing) - $1170	1