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April 4, 2021 18:07
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easy-taxonomy e77760e07c3167ef94f937365175d68b.assm.combined.fasta DB/uniref50-tax/UniRef50 e77760e07c3167ef94f937365175d68b.UniRef50 /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50 --tax-lineage 2 --majority 0.5 --vote-mode 1 --lca-mode 3 --orf-filter 1 --lca-ranks superkingdom,phylum,class,order,family,genus --threads 32 | |
MMseqs Version: 5daca424b162cc5fdf0b9cd151aebed86975cbf6 | |
ORF filter 1 | |
ORF filter e-value 100 | |
ORF filter sensitivity 2 | |
LCA mode 3 | |
Majority threshold 0.5 | |
Vote mode 1 | |
LCA ranks superkingdom,phylum,class,order,family,genus | |
Column with taxonomic lineage 2 | |
Compressed 0 | |
Threads 32 | |
Verbosity 3 | |
Taxon blacklist 12908:unclassified sequences,28384:other sequences | |
Substitution matrix nucl:nucleotide.out,aa:blosum62.out | |
Add backtrace false | |
Alignment mode 0 | |
Alignment mode 0 | |
Allow wrapped scoring false | |
E-value threshold 0.001 | |
Seq. id. threshold 0 | |
Min alignment length 0 | |
Seq. id. mode 0 | |
Alternative alignments 0 | |
Coverage threshold 0 | |
Coverage mode 0 | |
Max sequence length 65535 | |
Compositional bias 1 | |
Max reject 2147483647 | |
Max accept 2147483647 | |
Include identical seq. id. false | |
Preload mode 0 | |
Pseudo count a 1 | |
Pseudo count b 1.5 | |
Score bias 0 | |
Realign hits false | |
Realign score bias -0.2 | |
Realign max seqs 2147483647 | |
Gap open cost nucl:5,aa:11 | |
Gap extension cost nucl:2,aa:1 | |
Zdrop 40 | |
Seed substitution matrix nucl:nucleotide.out,aa:VTML80.out | |
Sensitivity 4 | |
k-mer length 0 | |
k-score 2147483647 | |
Alphabet size nucl:5,aa:21 | |
Max results per query 300 | |
Split database 0 | |
Split mode 2 | |
Split memory limit 0 | |
Diagonal scoring true | |
Exact k-mer matching 0 | |
Mask residues 1 | |
Mask lower case residues 0 | |
Minimum diagonal score 15 | |
Spaced k-mers 1 | |
Spaced k-mer pattern | |
Local temporary path | |
Rescore mode 0 | |
Remove hits by seq. id. and coverage false | |
Sort results 0 | |
Mask profile 1 | |
Profile E-value threshold 0.001 | |
Global sequence weighting false | |
Allow deletions false | |
Filter MSA 1 | |
Maximum seq. id. threshold 0.9 | |
Minimum seq. id. 0 | |
Minimum score per column -20 | |
Minimum coverage 0 | |
Select N most diverse seqs 1000 | |
Min codons in orf 30 | |
Max codons in length 32734 | |
Max orf gaps 2147483647 | |
Contig start mode 2 | |
Contig end mode 2 | |
Orf start mode 1 | |
Forward frames 1,2,3 | |
Reverse frames 1,2,3 | |
Translation table 1 | |
Translate orf 0 | |
Use all table starts false | |
Offset of numeric ids 0 | |
Create lookup 0 | |
Add orf stop false | |
Overlap between sequences 0 | |
Sequence split mode 1 | |
Header split mode 0 | |
Chain overlapping alignments 0 | |
Merge query 1 | |
Search type 0 | |
Search iterations 1 | |
Start sensitivity 4 | |
Search steps 1 | |
Exhaustive search mode false | |
Filter results during exhaustive search 0 | |
Strand selection 1 | |
LCA search mode false | |
Disk space limit 0 | |
MPI runner | |
Force restart with latest tmp false | |
Remove temporary files true | |
Report mode 0 | |
Alignment format 0 | |
Format alignment output query,target,fident,alnlen,mismatch,gapopen,qstart,qend,tstart,tend,evalue,bits | |
Database output false | |
First sequence as representative false | |
Target column 1 | |
Add full header false | |
Sequence source 0 | |
Database type 0 | |
Shuffle input database true | |
Createdb mode 1 | |
Write lookup file 0 | |
createdb e77760e07c3167ef94f937365175d68b.assm.combined.fasta /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/query --dbtype 0 --shuffle 1 --createdb-mode 1 --write-lookup 0 --id-offset 0 --compressed 0 -v 3 | |
Shuffle database cannot be combined with --createdb-mode 0 | |
We recompute with --shuffle 0 | |
Converting sequences | |
[Multiline fasta can not be combined with --createdb-mode 0 | |
We recompute with --createdb-mode 1 | |
Time for merging to query_h: 0h 0m 0s 0ms | |
Time for merging to query: 0h 0m 0s 0ms | |
[ | |
Time for merging to query_h: 0h 0m 0s 0ms | |
Time for merging to query: 0h 0m 0s 0ms | |
Database type: Nucleotide | |
Time for processing: 0h 0m 0s 12ms | |
Create directory /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/taxonomy_tmp | |
taxonomy /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/query DB/uniref50-tax/UniRef50 /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/result /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/taxonomy_tmp --orf-filter 1 --lca-mode 3 --tax-output-mode 2 --majority 0.5 --vote-mode 1 --lca-ranks superkingdom,phylum,class,order,family,genus --tax-lineage 2 --threads 32 --remove-tmp-files 1 | |
extractorfs /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/query /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/taxonomy_tmp/13584421311535647719/orfs_aa --min-length 30 --max-length 32734 --max-gaps 2147483647 --contig-start-mode 2 --contig-end-mode 2 --orf-start-mode 1 --forward-frames 1,2,3 --reverse-frames 1,2,3 --translation-table 1 --translate 1 --use-all-table-starts 0 --id-offset 0 --create-lookup 0 --threads 32 --compressed 0 -v 3 | |
[=================================================================] 2 0s 33ms | |
Time for merging to orfs_aa_h: 0h 0m 0s 1ms | |
Time for merging to orfs_aa: 0h 0m 0s 1ms | |
Time for processing: 0h 0m 0s 139ms | |
prefilter /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/taxonomy_tmp/13584421311535647719/orfs_aa DB/uniref50-tax/UniRef50 /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/taxonomy_tmp/13584421311535647719/orfs_pref --sub-mat nucl:nucleotide.out,aa:blosum62.out --seed-sub-mat nucl:nucleotide.out,aa:VTML80.out -s 2 -k 0 --k-score 2147483647 --alph-size nucl:5,aa:21 --max-seq-len 65535 --max-seqs 1 --split 0 --split-mode 2 --split-memory-limit 0 -c 0 --cov-mode 0 --comp-bias-corr 1 --diag-score 0 --exact-kmer-matching 0 --mask 1 --mask-lower-case 0 --min-ungapped-score 3 --add-self-matches 0 --spaced-kmer-mode 1 --db-load-mode 0 --pca 1 --pcb 1.5 --threads 32 --compressed 0 -v 3 | |
Query database size: 15 type: Aminoacid | |
Estimated memory consumption: 151G | |
Target database size: 43032414 type: Aminoacid | |
Index table k-mer threshold: 163 at k-mer size 7 | |
Index table: counting k-mers | |
[=================================================================] 43.03M 4m 2s 212ms | |
Index table: Masked residues: 327874438 | |
Index table: fill | |
[=================================================================] 43.03M 11m 49s 219ms | |
Index statistics | |
Entries: 10015666182 | |
DB size: 67075 MB | |
Avg k-mer size: 7.824739 | |
Top 10 k-mers | |
DFEQLPH 65289 | |
NVPGWSP 65148 | |
FRYAFPS 64963 | |
RYYVLGW 64817 | |
VDRLNTQ 63812 | |
WRLDFLN 62949 | |
TVDGDFS 62609 | |
NKTDFVQ 61859 | |
QDWVQIP 61316 | |
LDGAYVP 59471 | |
Time for index table init: 0h 16m 24s 811ms | |
Process prefiltering step 1 of 1 | |
k-mer similarity threshold: 163 | |
Starting prefiltering scores calculation (step 1 of 1) | |
Query db start 1 to 15 | |
Target db start 1 to 43032414 | |
[=================================================================] 15 0s 59ms | |
0.355339 k-mers per position | |
274 DB matches per sequence | |
0 overflows | |
0 queries produce too many hits (truncated result) | |
0 sequences passed prefiltering per query sequence | |
0 median result list length | |
15 sequences with 0 size result lists | |
Time for merging to orfs_pref: 0h 0m 0s 2ms | |
Time for processing: 0h 16m 45s 727ms | |
rescorediagonal /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/taxonomy_tmp/13584421311535647719/orfs_aa DB/uniref50-tax/UniRef50 /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/taxonomy_tmp/13584421311535647719/orfs_pref /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/taxonomy_tmp/13584421311535647719/orfs_aln --sub-mat nucl:nucleotide.out,aa:blosum62.out --rescore-mode 2 --wrapped-scoring 0 --filter-hits 0 -e 100 -c 0 -a 0 --cov-mode 0 --min-seq-id 0 --min-aln-len 0 --seq-id-mode 0 --add-self-matches 0 --sort-results 0 --db-load-mode 0 --threads 32 --compressed 0 -v 3 | |
[=================================================================] 15 0s 21ms | |
Time for merging to orfs_aln: 0h 0m 0s 2ms | |
Time for processing: 0h 0m 19s 666ms | |
createsubdb /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/taxonomy_tmp/13584421311535647719/orfs_aln.list /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/taxonomy_tmp/13584421311535647719/orfs_aa /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/taxonomy_tmp/13584421311535647719/orfs_filter --subdb-mode 1 -v 3 | |
Time for merging to orfs_filter: 0h 0m 0s 0ms | |
Time for processing: 0h 0m 0s 1ms | |
rmdb /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/taxonomy_tmp/13584421311535647719/orfs_filter_h -v 3 | |
Time for processing: 0h 0m 0s 0ms | |
createsubdb /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/taxonomy_tmp/13584421311535647719/orfs_aln.list /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/taxonomy_tmp/13584421311535647719/orfs_aa_h /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/taxonomy_tmp/13584421311535647719/orfs_filter_h --subdb-mode 1 -v 3 | |
Time for merging to orfs_filter_h: 0h 0m 0s 0ms | |
Time for processing: 0h 0m 0s 1ms | |
Create directory /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/taxonomy_tmp/13584421311535647719/tmp_taxonomy | |
taxonomy /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/taxonomy_tmp/13584421311535647719/orfs_filter DB/uniref50-tax/UniRef50 /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/taxonomy_tmp/13584421311535647719/orfs_tax /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/taxonomy_tmp/13584421311535647719/tmp_taxonomy --orf-filter 1 --lca-mode 3 --tax-output-mode 2 --majority 0.5 --vote-mode 1 --lca-ranks superkingdom,phylum,class,order,family,genus --tax-lineage 0 --threads 32 --alignment-mode 1 -e 1 --max-rejected 5 --max-accept 30 -s 2 --spaced-kmer-mode 1 --min-length 30 --max-length 32734 --orf-start-mode 1 --remove-tmp-files 1 | |
Create directory /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/taxonomy_tmp/13584421311535647719/tmp_taxonomy/4936078414881223269/tmp_hsp1 | |
search /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/taxonomy_tmp/13584421311535647719/orfs_filter DB/uniref50-tax/UniRef50 /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/taxonomy_tmp/13584421311535647719/tmp_taxonomy/4936078414881223269/first /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/taxonomy_tmp/13584421311535647719/tmp_taxonomy/4936078414881223269/tmp_hsp1 --alignment-mode 1 -e 1 --max-rejected 5 --max-accept 30 --threads 32 -s 2 --spaced-kmer-mode 1 --min-length 30 --max-length 32734 --orf-start-mode 1 --lca-search 1 --remove-tmp-files 1 | |
prefilter /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/taxonomy_tmp/13584421311535647719/orfs_filter DB/uniref50-tax/UniRef50 /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/taxonomy_tmp/13584421311535647719/tmp_taxonomy/4936078414881223269/tmp_hsp1/16551820070899017371/pref_0 --sub-mat nucl:nucleotide.out,aa:blosum62.out --seed-sub-mat nucl:nucleotide.out,aa:VTML80.out -k 0 --k-score 2147483647 --alph-size nucl:5,aa:21 --max-seq-len 65535 --max-seqs 300 --split 0 --split-mode 2 --split-memory-limit 0 -c 0 --cov-mode 0 --comp-bias-corr 1 --diag-score 1 --exact-kmer-matching 0 --mask 1 --mask-lower-case 0 --min-ungapped-score 15 --add-self-matches 0 --spaced-kmer-mode 1 --db-load-mode 0 --pca 1 --pcb 1.5 --threads 32 --compressed 0 -v 3 -s 2.0 | |
Query database size: 0 type: Aminoacid | |
Estimated memory consumption: 151G | |
Target database size: 43032414 type: Aminoacid | |
Index table k-mer threshold: 163 at k-mer size 7 | |
Index table: counting k-mers | |
[=================================================================] 43.03M 4m 11s 993ms | |
Index table: Masked residues: 327874438 | |
Index table: fill | |
[=================================================================] 43.03M 11m 31s 69ms | |
Index statistics | |
Entries: 10015666182 | |
DB size: 67075 MB | |
Avg k-mer size: 7.824739 | |
Top 10 k-mers | |
DFEQLPH 65289 | |
NVPGWSP 65148 | |
FRYAFPS 64963 | |
RYYVLGW 64817 | |
VDRLNTQ 63812 | |
WRLDFLN 62949 | |
TVDGDFS 62609 | |
NKTDFVQ 61859 | |
QDWVQIP 61316 | |
LDGAYVP 59471 | |
Time for index table init: 0h 16m 9s 488ms | |
Time for processing: 0h 16m 22s 538ms | |
lcaalign /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/taxonomy_tmp/13584421311535647719/orfs_filter DB/uniref50-tax/UniRef50 /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/taxonomy_tmp/13584421311535647719/tmp_taxonomy/4936078414881223269/tmp_hsp1/16551820070899017371/pref_0 /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/taxonomy_tmp/13584421311535647719/tmp_taxonomy/4936078414881223269/first --sub-mat nucl:nucleotide.out,aa:blosum62.out -a 0 --alignment-mode 1 --alignment-output-mode 0 --wrapped-scoring 0 -e 1 --min-seq-id 0 --min-aln-len 0 --seq-id-mode 0 --alt-ali 0 -c 0 --cov-mode 0 --max-seq-len 65535 --comp-bias-corr 1 --max-rejected 5 --max-accept 30 --add-self-matches 0 --db-load-mode 0 --pca 1 --pcb 1.5 --score-bias 0 --realign 0 --realign-score-bias -0.2 --realign-max-seqs 2147483647 --gap-open nucl:5,aa:11 --gap-extend nucl:2,aa:1 --zdrop 40 --threads 32 --compressed 0 -v 3 | |
Input /dev/shm/tmp/contig-taxonomy/e77760e07c3167ef94f937365175d68b.tax.UniRef50/12595762918832005520/taxonomy_tmp/13584421311535647719/tmp_taxonomy/4936078414881223269/tmp_hsp1/16551820070899017371/pref_0 does not exist | |
Error: Alignment died | |
Error: First search died | |
Error: taxonomy died | |
Error: Search died |
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