Created
October 14, 2025 15:07
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Parsing ome.tiff for getting channels
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| import tifffile | |
| file_name = 'laulab_tma1_file.ome.tiff' | |
| with tifffile.TiffFile(file_name) as tif: | |
| img = tif.asarray() | |
| print(f"Image shape: {img.shape}") | |
| print(f"Image dtype: {img.dtype}") | |
| print(f"Number of pages: {len(tif.pages)}") | |
| # Check if OME metadata exists | |
| if tif.ome_metadata: | |
| print("\nOME metadata found") | |
| print(tif.ome_metadata[:500]) | |
| # Parse OME metadata to get channel names | |
| if tif.ome_metadata: | |
| import xml.etree.ElementTree as ET | |
| try: | |
| root = ET.fromstring(tif.ome_metadata) | |
| ns = {'ome': 'http://www.openmicroscopy.org/Schemas/OME/2016-06'} | |
| # Find all channels | |
| channels = root.findall('.//ome:Channel', ns) | |
| print(f"\n=== FOUND {len(channels)} CHANNELS/PROTEINS ===") | |
| for i, ch in enumerate(channels): | |
| name = ch.get('Name', f'Channel_{i}') | |
| ch_id = ch.get('ID', 'N/A') | |
| print(f" {i}: {name} (ID: {ch_id})") | |
| # Also check image dimensions | |
| pixels = root.find('.//ome:Pixels', ns) | |
| if pixels is not None: | |
| print(f"\nImage dimensions from OME:") | |
| print(f" SizeC (channels): {pixels.get('SizeC')}") | |
| print(f" SizeX: {pixels.get('SizeX')}") | |
| print(f" SizeY: {pixels.get('SizeY')}") | |
| print(f" SizeZ: {pixels.get('SizeZ', '1')}") | |
| except Exception as e: | |
| print(f"Could not parse OME XML: {e}") |
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