process_xlsx.py

from tkinter import *
from openpyxl import load_workbook

root = Tk()
root.title('Врачебная программа (alfa)')
root.geometry("350x200")

entry_name_1 = Entry(width=26, bd=5)
entry_name_1.place(x=70, y=10)
label_name_1 = Label(width=7, bd=5, text="Entry_1")
label_name_1.place(x=5, y=10)

entry_name_2 = Entry(width=26, bd=5)
entry_name_2.place(x=70, y=45)
label_name_2 = Label(width=7, bd=5, text="Entry_2")
label_name_2.place(x=5, y=45)


def process_xlsx():
    workbook = load_workbook('template.xlsx')
    worksheet = workbook['Лист1']

    worksheet['B3'] = entry_name_1.get()
    worksheet['C5'] = entry_name_2.get()
    workbook.save('result.xlsx')


btn1 = Button(text='Сформировать документ', command=process_xlsx)
btn1.place(x=40, y=100)

root.mainloop()