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@iracooke
iracooke / README.md
Last active October 28, 2022 05:18
NCBI TSA Submission Guide

Steps to submit to TSA

If you have a transcriptome that has been assembled from shotgun reads the TSA (Transcriptome Shotgun Assembly) database is a good place to put it so that it can be widely accessed.

This guide assumes that you simply want to submit the assembled sequences from your transcriptome without annotations. NCBI sets a high bar for inclusion of annotations so for most non-model organisms they are probably not going to meet the criteria.

To create a TSA submission take a look at the ncbi guidelines. This gist is based on those guidelines.

Register BioProject

@christofluethi
christofluethi / gist:646ae60d797a46a706a5
Last active April 1, 2024 22:10
Convert m4a to mp3 on OS X command line using ffmpeg
brew update
brew link yasm
brew link x264
brew link lame
brew link xvid
brew install ffmpeg
ffmpeg wiki:
https://trac.ffmpeg.org/wiki/Encode/MP3