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| bedtools genomecov -ibam bam/por1_fastq.bam -bga | awk '$4==0' | bedtools intersect -a - -b ~/miniconda3/envs/dev/share/tbprofiler/tbdb.bed | bedtools subtract -a - -b test2.vcf |
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bedtools coverage -b bam/ERR027452.bam -a ~/miniconda3/envs/dev/share/tbprofiler/tbdb.bed -d | awk '$9=$7+$2' | tr ' ' '\t' | awk '$8<10'