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Hacks to load an hdf5 biom file and be able to add it to metagenomeSeq or phyloseq
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source("http://bioconductor.org/biocLite.R") | |
biocLite(c("rhdf5","biom")) | |
library(rhdf5) | |
library(biom) | |
# This generates the matrix columns-wise | |
generate_matrix <- function(x){ | |
indptr = x$sample$matrix$indptr+1 | |
indices = x$sample$matrix$indices+1 | |
data = x$sample$matrix$data | |
nr = length(x$observation$ids) | |
counts = sapply(2:length(indptr),function(i){ | |
x = rep(0,nr) | |
seq = indptr[i-1]:(indptr[i]-1) | |
x[indices[seq]] = data[seq] | |
x | |
}) | |
rownames(counts) = x$observation$ids | |
colnames(counts) = x$sample$ids | |
# I wish this next line wasn't necessary | |
lapply(1:nrow(counts),function(i){ | |
counts[i,] | |
}) | |
} | |
generate_metadata <- function(x){ | |
metadata = x$metadata | |
metadata = lapply(1:length(x$ids),function(i){ | |
id_metadata = lapply(metadata,function(j){ | |
if(length(dim(j))>1){ as.vector(j[,i,drop=FALSE]) } | |
else{ j[i] } | |
}) | |
list(id = x$ids[i],metadata=id_metadata) | |
}) | |
return(metadata) | |
} | |
namedList <- function(...) { | |
L <- list(...) | |
snm <- sapply(substitute(list(...)),deparse)[-1] | |
if (is.null(nm <- names(L))) nm <- snm | |
if (any(nonames <- nm=="")) nm[nonames] <- snm[nonames] | |
setNames(L,nm) | |
} | |
read_hdf5_biom<-function(file_input){ | |
x = h5read(file_input,"/",read.attributes = TRUE) | |
data = generate_matrix(x) | |
rows = generate_metadata(x$observation) | |
columns = generate_metadata(x$sample) | |
shape = c(length(data),length(data[[1]])) # dim(data) | |
# Experimental -- need to actually load these from file | |
id = attr(x,"id") | |
vs = attr(x,"format-version") | |
format = sprintf("Biological Observation Matrix %s.%s",vs[1],vs[2]) | |
format_url = attr(x,"format-url") | |
type = "OTU table" | |
#type=attr(x,"type") | |
generated_by = attr(x,"generated-by") | |
date = attr(x,"creation-date") | |
matrix_type = "dense" | |
matrix_element_type = "int" | |
namedList(id,format,format_url,type,generated_by,date,matrix_type,matrix_element_type, | |
rows,columns,shape,data) | |
} | |
y = read_hdf5_biom("rich_sparse_otu_table_hdf5.biom") | |
# This won't work now if the type is not acceptable. | |
biom(y) |
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