gistfile1.txt

#!/usr/env/python

import hashlib, os

def md5(fname):
    try:
        hash_md5 = hashlib.md5()
        with open(fname, "rb") as f:
            for chunk in iter(lambda: f.read(4096), b""):
                hash_md5.update(chunk)
        return hash_md5.hexdigest()
    except IsADirectoryError:
        return None

dir0 = 'DIRECTORY_GOES_HERE'
dir1 = 'DIRECTORY_GOES_HERE''

hashes0 = {md5(os.path.join(dir0,filename)) for filename in os.listdir(dir0)}
hashes1 = {md5(os.path.join(dir1,filename)) for filename in os.listdir(dir1)}

assert hashes0 == hashes1