itsyBitsyPx.js

var gif;
fs.readFile('./trans.gif', function (err, data)
{
	if (err) throw err;
	gif = new Buffer(data, 'binary');
});

http.createServer(function (req, res)
{
	res.writeHead(200, {'Content-Type' : 'image/gif'});
	req.setEncoding('binary');
	res.write(gif);
	res.end();

	// Do db magic after the request is sent.
}).listen(80);