ex.rb

Protocol.new :title => "bacterial transformation" do

  plasmid = Storage.get "demo:isolated-plasmid"

  vial = Container.new(:centrifuge_15ml)
                  .add(5.uL, :from => plasmid)
                  .cool(277.kelvin, :for => 10.minutes)

  tube = Container.new(:culture_tube).add(0.5.mL, Chemical.find(:media, :lysogeny_media))

  plates = [Plate.new(:media => :lysogeny_media),
            Plate.new(:media => :lysogeny_media)]
  plates.map { |plate|
    plate.add 2.mL, Chemical.find(:reagent, :kanamycin)
  }

  iptg    = plates[0].add 25.uL, Chemical.find(:reagent, :IPTG)
  control = plates[1]

  plates.map { |plate|
    plate.incubate 2.hours, 298.kelvin, 5, 5
  }

  tube.add :all, :from => vial
  tube.incubate 2.hours, 37.degC, 5, 5

  plates.each_with_index { |plate, i|
    plate.add 50.uL, :from => tube
    plate.incubate 12.hours, 298.kelvin, 5, 5
    Storage.put "demo:competent-plate-#{i}", plate, :common
  }

end