This gist calls two numbers from a given Twilio number and then connects the callers to a conference.

connect_conf.py

from flask import Flask

from twilio.rest import TwilioRestClient

from twilio import twiml

app = Flask(__name__)

client = TwilioRestClient(SID, token)

client.calls.create(to="FIRST_PHONE_NUMBER", from_="YOUR_TWILIO_NUMBER", url='http://URL/conference')
client.calls.create(to="SECOND_PHONE_NUMBER", from_="YOUR_TWILIO_NUMBER", url='http://URL/conference')

@app.route('/conference', methods=['GET', 'POST'])
def conference_call():
	res = twiml.Response()
	with res.dial() as dial:
		dial.conference("My Conf")
	return res.toxml()


if __name__ == "__main__":
	app.run()