gistfile1.py

In [1]: from random import randint

In [2]: lines = [randint(0, 42) for _ in range(10000)]

In [3]: %%timeit
   ...: for _ in lines[::-1]: pass
   ...: 
10000 loops, best of 3: 149 µs per loop

In [4]: %%timeit
for _ in reversed(lines): pass
   ...: 
10000 loops, best of 3: 120 µs per loop