Some simple Python scripts to generate every possible an n x n image of black and transparent pixels. The other one merges the images together

images.py

# Nathan Craddock 2016

from PIL import Image
import random
import os
from subprocess import run
from itertools import product

size = (4, 4)
colors = [(0, 0, 0, 0), (0, 0, 0, 255)]
filepath = "images"

def create_image(name, pattern):
	img = Image.new('RGBA', size)

	i = 0
	pixels = img.load()
	for y in range(img.size[1]):
		for x in range(img.size[0]):
			if pattern[i]:
				col = colors[1]
			else:
				col = colors[0]
			pixels[x, y] = col			
			i += 1

	img.save(os.path.join(filepath, str(name)) + '.png')

patterns = product([False, True], repeat=size[0] * size[1])

image_name = 1
for p in patterns:

	create_image(image_name, list(p))
	image_name += 1

merge_images.py

from PIL import Image
import os

size = (1024, 1024)
filepath = "images"

img = Image.new('RGBA', size)
pixels = img.load()

i = 1
for y in range(256):
	for x in range(256):
		# We are at the coords of a specific image, time to add the pixels
		
		part = Image.open(os.path.join(filepath, str(i)) + '.png')
		part_pixels = part.load()

		for py in range(part.size[1]):
			for px in range(part.size[0]):
				pixels[x * 4 + px, y * 4 + py] = part_pixels[px, py]
		
		i += 1

img.save('final_merge.png')