Created
November 2, 2018 18:01
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It is useful to have markers with MAF >1% in a given 1000G population, for filtering of combined genotype data, LDAK, etc.
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| #List 1000g Europeans | |
| awk '{if ($3 == "EUR") print $1}' integrated_call_samples_v3.20130502.ALL.panel > 1000g_europeans.subjects | |
| for i in 22 # {1..22} | |
| do | |
| vcf-subset -c 1000g_europeans.subjects ALL.chr"$i".phase3_shapeit2_mvncall_integrated_v5a.20130502.genotypes.vcf.gz | fill-an-ac | gzip -c > CEU.chr"$i".phase1.vcf.gz | |
| done | |
| #Get marker info for all markers. Only take rs markers that are di-allelic | |
| for i in {1..22} | |
| do | |
| #zcat ALL.chr"$i".phase3_shapeit2_mvncall_integrated_v5a.20130502.genotypes.vcf.gz | awk 'NR>=253{print $3,$8}' | grep -v MULTI_ALLELIC | grep rs | gzip -c > info_ALL.chr"$i".phase3_shapeit2_mvncall_integrated_v5a.20130502.genotypes.vcf.gz | |
| # zcat info_ALL.chr"$i".phase3_shapeit2_mvncall_integrated_v5a.20130502.genotypes.vcf.gz | awk -F ";|\ " ' {print $1,$9}' | sed 's/AFR_AF=//g' | awk '{if ($2 >= 0.01 && $2 <= 0.99) print $1}' | gzip -c > afmaf01_ALL.chr"$i".phase3_shapeit2_mvncall_integrated_v5a.20130502.genotypes.vcf.gz | |
| # zcat info_ALL.chr"$i".phase3_shapeit2_mvncall_integrated_v5a.20130502.genotypes.vcf.gz | awk -F ";|\ " ' {print $1,$10}' | sed 's/EUR_AF=//g' | awk '{if ($2 >= 0.01 && $2 <= 0.99) print $1}' | gzip -c > eurmaf01_ALL.chr"$i".phase3_shapeit2_mvncall_integrated_v5a.20130502.genotypes.vcf.gz | |
| zcat info_ALL.chr"$i".phase3_shapeit2_mvncall_integrated_v5a.20130502.genotypes.vcf.gz | awk -F ";|\ " ' {print $1,$3}' | sed 's/AF=//g' | awk '{if ($2 >= 0.01 && $2 <= 0.99) print $1}' | gzip -c > allmaf01_ALL.chr"$i".phase3_shapeit2_mvncall_integrated_v5a.20130502.genotypes.vcf.gz | |
| done | |
| zcat afmaf01_ALL.chr*.phase3_shapeit2_mvncall_integrated_v5a.20130502.genotypes.vcf.gz | gzip -c > afrmaf01_ALL.allchr.phase3_shapeit2_mvncall_integrated_v5a.20130502.genotypes.vcf.gz | |
| zcat eurmaf01_ALL.chr*.phase3_shapeit2_mvncall_integrated_v5a.20130502.genotypes.vcf.gz | gzip -c > eurmaf01_ALL.allchr.phase3_shapeit2_mvncall_integrated_v5a.20130502.genotypes.vcf.gz | |
| zcat allmaf01_ALL.chr*.phase3_shapeit2_mvncall_integrated_v5a.20130502.genotypes.vcf.gz | gzip -c > allmaf01_ALL.allchr.phase3_shapeit2_mvncall_integrated_v5a.20130502.genotypes.vcf.gz | |
| #Some markers have multiple names, annoyingly. Fuck it, just print name . These mave multiple AFs, delimited by , | |
| #Multi allelic markers have commas to delimit AF of the minor variants. We won't deal with that shit here | |
| #Fuck it and just get the SNPS | |
| zcat info_ALL.chr"$i".phase3_shapeit2_mvncall_integrated_v5a.20130502.genotypes.vcf.gz | grep "EUR_AF=0.0209,0.0149" -w -m1 |
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| #Get marker alleles for all markers | |
| for i in {1..22} | |
| do | |
| zcat ALL.chr"$i".phase3_shapeit2_mvncall_integrated_v5a.20130502.genotypes.vcf.gz | awk 'NR>=253{print $1,$2,$3,$4,$5,$8}' | gzip -c > acs_ALL.chr"$i".phase3_shapeit2_mvncall_integrated_v5a.20130502.genotypes.vcf.gz | |
| done |
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