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## RNA-seq analysis with DESeq2 | |
## Stephen Turner, @genetics_blog | |
# RNA-seq data from GSE52202 | |
# http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=gse52202. All patients with | |
# ALS, 4 with C9 expansion ("exp"), 4 controls without expansion ("ctl") | |
# Import & pre-process ---------------------------------------------------- | |
# Import data from featureCounts |
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# assumes you have SSH'ed and qlogin'ed | |
cd thu | |
cd mydata | |
# slide 5 | |
curl https://s3.amazonaws.com/gemini-tutorials/trio.trim.vep.vcf.gz > trio.trim.vep.vcf.gz | |
curl https://s3.amazonaws.com/gemini-tutorials/dominant.ped > dominant.ped | |
gemini load --cores 2 \ | |
-v trio.trim.vep.vcf.gz \ | |
-t VEP \ |
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-- [[ Cheatsheet for LUA from http://www.newthinktank.com/2015/06/learn-lua-one-video/]] | |
-- Prints to the screen (Can end with semicolon) | |
print("Hello World") | |
--[[ | |
Multiline comment | |
]] | |
-- Variable names can't start with a number, but can contain letters, numbers |