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example
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process cutadapt { | |
tag "${sampleid}" | |
container 'quay.io/biocontainers/cutadapt:1.18--py36_0' | |
publishDir "$params.outdir/cutadapt", mode: 'copy' | |
input: | |
tuple sampleid, path(read1), path(read2) from ch_samples_cutadapt | |
output: | |
tuple sampleid, path("${sampleid}_R1.cutadapt.fastq.gz"), path("${sampleid}_R2.cutadapt.fastq.gz") into ( ch_cutadapt_fastqc, ch_cutadapt_star, ch_cutadapt_for_sortmerna ) | |
path("${sampleid}_cutadapt.txt") into ch_cutadapt_multiqc | |
path ("version_cutadapt.txt") into ch_version_cutadapt | |
script: | |
""" | |
cutadapt -m 25 -q 20 \ | |
-a $params.clip_forward_adapter \ | |
-A $params.clip_reverse_adapter \ | |
-o ${sampleid}_R1.cutadapt.fastq.gz -p ${sampleid}_R2.cutadapt.fastq.gz \ | |
--cores $task.cpus \ | |
$read1 $read2 > ${sampleid}_cutadapt.txt 2>&1 | |
cutadapt --version | sed 's/^/ <br>cutadapt /' > version_cutadapt.txt | |
""" | |
} |
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