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@pcantalupo
Created April 16, 2020 19:40
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$ nextflow run nf-core/rnaseq -profile docker --aligner hisat2 -r 1.4.2 --reads 'data/*_{1,2}_10k.fastq' --reverseStranded --max_cpus 6 --max_memory 32 -c config_bkv.txt
NOTE: Nextflow is not tested with Java 13.0.2 -- It's recommended the use of version 8 up to 12
N E X T F L O W ~ version 20.01.0
Launching `nf-core/rnaseq` [nostalgic_solvay] - revision: 3b6df9bd10 [1.4.2]
----------------------------------------------------
,--./,-.
___ __ __ __ ___ /,-._.--~'
|\ | |__ __ / ` / \ |__) |__ } {
| \| | \__, \__/ | \ |___ \`-._,-`-,
`._,._,'
nf-core/rnaseq v1.4.2
----------------------------------------------------
Pipeline Release : 1.4.2
Run Name : nostalgic_solvay
Reads : data/*_{1,2}_10k.fastq
Data Type : Paired-End
Strandedness : Reverse
Trimming : 5'R1: 0 / 5'R2: 0 / 3'R1: 0 / 3'R2: 0 / NextSeq Trim: 0
Aligner : HISAT2
Fasta Ref : refs/bkv.fa
GTF Annotation : refs/GCF_000837865.1_ViralProj14074_genomic.gtf
Remove Ribosomal RNA: false
Biotype GTF field : gene_biotype
Save prefs : Ref Genome: No / Trimmed FastQ: No / Alignment intermediates: No
Max Resources : 32 memory, 6 cpus, 10d time per job
Container : docker - nfcore/rnaseq:1.4.2
Output dir : ./results
Launch dir : /Users/pgc92/projects/nextflow_nfcore_rnaseq/bkv
Working dir : /Users/pgc92/projects/nextflow_nfcore_rnaseq/bkv/work
Script dir : /Users/pgc92/.nextflow/assets/nf-core/rnaseq
User : pgc92
Config Profile : docker
----------------------------------------------------
executor > local (9)
[44/b80885] process > get_software_versions [100%] 1 of 1 ✔
[23/1028e5] process > makeBED12 [100%] 1 of 1 ✔
[ac/b75022] process > makeHisatSplicesites [100%] 1 of 1 ✔
[4c/51c4d2] process > makeHISATindex [100%] 1 of 1 ✔
[5c/15cd5a] process > fastqc [100%] 1 of 1 ✔
[25/9537dc] process > trim_galore [100%] 1 of 1 ✔
[dc/e167cc] process > hisat2Align [100%] 1 of 1 ✔
[85/1b49a2] process > hisat2_sortOutput [100%] 1 of 1, failed: 1 ✘
[- ] process > rseqc -
[- ] process > preseq -
[- ] process > markDuplicates -
[- ] process > qualimap -
[- ] process > dupradar -
[- ] process > featureCounts -
[- ] process > merge_featureCounts -
[- ] process > stringtieFPKM -
[- ] process > sample_correlation -
[- ] process > multiqc -
[30/9d40a3] process > output_documentation [100%] 1 of 1 ✔
[HISAT2 index build] Available memory: 32 B
[HISAT2 index build] Less than 200 GB available, so NOT using splice sites and exons in HISAT2 index.
[HISAT2 index build] Use --hisat_build_memory [small number] to skip this check.
[0;35m[nf-core/rnaseq] Pipeline completed with errors
WARN: To render the execution DAG in the required format it is required to install Graphviz -- See http://www.graphviz.org for more info.
Error executing process > 'hisat2_sortOutput (SRR11074361_1_10k)'
Caused by:
Process `hisat2_sortOutput (SRR11074361_1_10k)` terminated with an error exit status (1)
Command executed:
samtools sort \
SRR11074361_1_10k.bam \
-@ 4 -m-1499999992 \
-o SRR11074361_1_10k.sorted.bam
samtools index SRR11074361_1_10k.sorted.bam
Command exit status:
1
Command output:
(empty)
Command error:
samtools sort: couldn't allocate memory for bam_mem
Work dir:
/Users/pgc92/projects/nextflow_nfcore_rnaseq/bkv/work/85/1b49a27565635a24e82d8dd1a5d9e6
Tip: view the complete command output by changing to the process work dir and entering the command `cat .command.out`
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