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April 16, 2020 19:40
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| $ nextflow run nf-core/rnaseq -profile docker --aligner hisat2 -r 1.4.2 --reads 'data/*_{1,2}_10k.fastq' --reverseStranded --max_cpus 6 --max_memory 32 -c config_bkv.txt | |
| NOTE: Nextflow is not tested with Java 13.0.2 -- It's recommended the use of version 8 up to 12 | |
| N E X T F L O W ~ version 20.01.0 | |
| Launching `nf-core/rnaseq` [nostalgic_solvay] - revision: 3b6df9bd10 [1.4.2] | |
| ---------------------------------------------------- | |
| ,--./,-. | |
| ___ __ __ __ ___ /,-._.--~' | |
| |\ | |__ __ / ` / \ |__) |__ } { | |
| | \| | \__, \__/ | \ |___ \`-._,-`-, | |
| `._,._,' | |
| nf-core/rnaseq v1.4.2 | |
| ---------------------------------------------------- | |
| Pipeline Release : 1.4.2 | |
| Run Name : nostalgic_solvay | |
| Reads : data/*_{1,2}_10k.fastq | |
| Data Type : Paired-End | |
| Strandedness : Reverse | |
| Trimming : 5'R1: 0 / 5'R2: 0 / 3'R1: 0 / 3'R2: 0 / NextSeq Trim: 0 | |
| Aligner : HISAT2 | |
| Fasta Ref : refs/bkv.fa | |
| GTF Annotation : refs/GCF_000837865.1_ViralProj14074_genomic.gtf | |
| Remove Ribosomal RNA: false | |
| Biotype GTF field : gene_biotype | |
| Save prefs : Ref Genome: No / Trimmed FastQ: No / Alignment intermediates: No | |
| Max Resources : 32 memory, 6 cpus, 10d time per job | |
| Container : docker - nfcore/rnaseq:1.4.2 | |
| Output dir : ./results | |
| Launch dir : /Users/pgc92/projects/nextflow_nfcore_rnaseq/bkv | |
| Working dir : /Users/pgc92/projects/nextflow_nfcore_rnaseq/bkv/work | |
| Script dir : /Users/pgc92/.nextflow/assets/nf-core/rnaseq | |
| User : pgc92 | |
| Config Profile : docker | |
| ---------------------------------------------------- | |
| executor > local (9) | |
| [44/b80885] process > get_software_versions [100%] 1 of 1 ✔ | |
| [23/1028e5] process > makeBED12 [100%] 1 of 1 ✔ | |
| [ac/b75022] process > makeHisatSplicesites [100%] 1 of 1 ✔ | |
| [4c/51c4d2] process > makeHISATindex [100%] 1 of 1 ✔ | |
| [5c/15cd5a] process > fastqc [100%] 1 of 1 ✔ | |
| [25/9537dc] process > trim_galore [100%] 1 of 1 ✔ | |
| [dc/e167cc] process > hisat2Align [100%] 1 of 1 ✔ | |
| [85/1b49a2] process > hisat2_sortOutput [100%] 1 of 1, failed: 1 ✘ | |
| [- ] process > rseqc - | |
| [- ] process > preseq - | |
| [- ] process > markDuplicates - | |
| [- ] process > qualimap - | |
| [- ] process > dupradar - | |
| [- ] process > featureCounts - | |
| [- ] process > merge_featureCounts - | |
| [- ] process > stringtieFPKM - | |
| [- ] process > sample_correlation - | |
| [- ] process > multiqc - | |
| [30/9d40a3] process > output_documentation [100%] 1 of 1 ✔ | |
| [HISAT2 index build] Available memory: 32 B | |
| [HISAT2 index build] Less than 200 GB available, so NOT using splice sites and exons in HISAT2 index. | |
| [HISAT2 index build] Use --hisat_build_memory [small number] to skip this check. | |
| [0;35m[nf-core/rnaseq] Pipeline completed with errors | |
| WARN: To render the execution DAG in the required format it is required to install Graphviz -- See http://www.graphviz.org for more info. | |
| Error executing process > 'hisat2_sortOutput (SRR11074361_1_10k)' | |
| Caused by: | |
| Process `hisat2_sortOutput (SRR11074361_1_10k)` terminated with an error exit status (1) | |
| Command executed: | |
| samtools sort \ | |
| SRR11074361_1_10k.bam \ | |
| -@ 4 -m-1499999992 \ | |
| -o SRR11074361_1_10k.sorted.bam | |
| samtools index SRR11074361_1_10k.sorted.bam | |
| Command exit status: | |
| 1 | |
| Command output: | |
| (empty) | |
| Command error: | |
| samtools sort: couldn't allocate memory for bam_mem | |
| Work dir: | |
| /Users/pgc92/projects/nextflow_nfcore_rnaseq/bkv/work/85/1b49a27565635a24e82d8dd1a5d9e6 | |
| Tip: view the complete command output by changing to the process work dir and entering the command `cat .command.out` |
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