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Canonical Correspondence Analysis in R using the vegan library
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library(vegan) | |
library(labdsv) | |
data(varespec) | |
data(varechem) | |
vare.cca <- cca(varespec ~ Baresoil+Humdepth+pH+N+P+K+Ca+Mg+S+Al+Fe, data=varechem) | |
vare.cca | |
plot(vare.cca) | |
summary(vare.cca) | |
// See also http://ecology.msu.montana.edu/labdsv/R/labs/lab12/lab12.html |
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> vare.cca | |
Call: cca(formula = varespec ~ Baresoil + Humdepth + pH + N + P + K + Ca + Mg + S + Al + Fe, data = varechem) | |
Inertia Proportion Rank | |
Total 2.0832 1.0000 | |
Constrained 1.2154 0.5834 11 | |
Unconstrained 0.8678 0.4166 12 | |
Inertia is mean squared contingency coefficient | |
Eigenvalues for constrained axes: | |
CCA1 CCA2 CCA3 CCA4 CCA5 CCA6 CCA7 CCA8 CCA9 CCA10 CCA11 | |
0.410865 0.255619 0.146158 0.131515 0.091575 0.078525 0.060261 0.019134 0.008944 0.006849 0.005946 | |
Eigenvalues for unconstrained axes: | |
CA1 CA2 CA3 CA4 CA5 CA6 CA7 CA8 CA9 CA10 CA11 CA12 | |
0.253513 0.151934 0.126683 0.093370 0.075393 0.049632 0.037929 0.026435 0.021758 0.014304 0.009052 0.007806 | |
> plot(vare.cca) | |
> summary(vare.cca) | |
Call: | |
cca(formula = varespec ~ Baresoil + Humdepth + pH + N + P + K + Ca + Mg + S + Al + Fe, data = varechem) | |
Partitioning of mean squared contingency coefficient: | |
Inertia Proportion | |
Total 2.0832 1.0000 | |
Constrained 1.2154 0.5834 | |
Unconstrained 0.8678 0.4166 | |
Eigenvalues, and their contribution to the mean squared contingency coefficient | |
Importance of components: | |
CCA1 CCA2 CCA3 CCA4 CCA5 CCA6 CCA7 CCA8 | |
Eigenvalue 0.4109 0.2556 0.14616 0.13152 0.09158 0.07852 0.06026 0.01913 | |
Proportion Explained 0.1972 0.1227 0.07016 0.06313 0.04396 0.03769 0.02893 0.00918 | |
Cumulative Proportion 0.1972 0.3199 0.39009 0.45322 0.49718 0.53488 0.56381 0.57299 | |
CCA9 CCA10 CCA11 CA1 CA2 CA3 CA4 CA5 | |
Eigenvalue 0.008944 0.006849 0.005946 0.2535 0.15193 0.12668 0.09337 0.07539 | |
Proportion Explained 0.004290 0.003290 0.002850 0.1217 0.07293 0.06081 0.04482 0.03619 | |
Cumulative Proportion 0.577280 0.580570 0.583430 0.7051 0.77805 0.83886 0.88368 0.91988 | |
CA6 CA7 CA8 CA9 CA10 CA11 CA12 | |
Eigenvalue 0.04963 0.03793 0.02643 0.02176 0.01430 0.009052 0.007806 | |
Proportion Explained 0.02382 0.01821 0.01269 0.01044 0.00687 0.004350 0.003750 | |
Cumulative Proportion 0.94370 0.96191 0.97460 0.98504 0.99191 0.996250 1.000000 | |
Accumulated constrained eigenvalues | |
Importance of components: | |
CCA1 CCA2 CCA3 CCA4 CCA5 CCA6 CCA7 CCA8 | |
Eigenvalue 0.4109 0.2556 0.1462 0.1315 0.09158 0.07852 0.06026 0.01913 | |
Proportion Explained 0.3381 0.2103 0.1203 0.1082 0.07535 0.06461 0.04958 0.01574 | |
Cumulative Proportion 0.3381 0.5484 0.6686 0.7768 0.85218 0.91679 0.96637 0.98211 | |
CCA9 CCA10 CCA11 | |
Eigenvalue 0.008944 0.006849 0.005946 | |
Proportion Explained 0.007360 0.005630 0.004890 | |
Cumulative Proportion 0.989470 0.995110 1.000000 | |
Scaling 2 for species and site scores | |
* Species are scaled proportional to eigenvalues | |
* Sites are unscaled: weighted dispersion equal on all dimensions | |
Species scores | |
CCA1 CCA2 CCA3 CCA4 CCA5 CCA6 | |
Cal.vul 0.2579826 -0.80872 1.94560 -0.35626 0.697113 -0.74258 | |
Emp.nig -0.2704679 -0.05299 -0.14437 -0.32584 -0.170125 -0.04192 | |
Led.pal -0.9762750 -0.37214 -0.06644 -0.75402 -0.697058 0.06306 | |
Vac.myr -1.3055169 -0.11479 0.17137 0.27104 -0.599337 0.07048 | |
Vac.vit -0.1929037 0.08757 -0.21288 -0.35303 -0.066669 -0.10932 | |
Pin.syl 0.2380445 0.22966 0.05686 -0.39593 -0.019382 0.07840 | |
Des.fle -1.4062903 0.38398 0.39272 0.39030 -0.101391 0.30965 | |
Bet.pub -0.5960443 -0.95617 -0.72698 -1.67618 -1.588060 -0.32047 | |
Vac.uli 0.3926825 -0.94198 -0.39399 0.58151 -0.228095 -0.14642 | |
Dip.mon 0.0229110 -0.49673 -0.89897 -0.40174 -0.174258 -0.26730 | |
Dic.sp -0.4868957 0.65851 -1.26929 0.02094 1.419951 -0.57922 | |
Dic.fus -0.4861256 -0.47490 0.52892 -0.51872 0.229919 0.59969 | |
Dic.pol -0.3223530 -0.20367 -1.05718 -0.83490 -0.119395 -0.73626 | |
Hyl.spl -1.8827323 0.65465 0.49959 1.53295 -0.308169 0.16247 | |
Ple.sch -0.9252145 0.28944 0.09530 0.27152 0.053829 -0.02879 | |
Pol.pil 0.2594323 -0.52462 -0.49858 0.08717 0.163650 0.43618 | |
Pol.jun -0.7366157 0.12464 -0.27517 -0.14649 -0.314192 0.31794 | |
Pol.com -0.9458638 -0.14755 -0.27373 -0.71188 -0.855045 -0.28475 | |
Poh.nut -0.0348872 0.21735 -0.33522 -0.36923 0.179971 -0.30020 | |
Pti.cil -0.5533110 -0.80045 -0.73937 -1.41351 -1.316239 -0.52927 | |
Bar.lyc -0.5655746 -1.05360 -0.79808 -1.74760 -1.740288 -0.58626 | |
Cla.arb 0.3082534 -0.56549 -0.03583 0.08219 -0.096060 -0.13535 | |
Cla.ran 0.4695611 -0.53152 -0.16623 0.29331 0.037571 0.13895 | |
Cla.ste 0.7627157 0.70289 0.12199 -0.02713 -0.053315 0.10624 | |
Cla.unc -0.0840257 -0.04106 -0.16775 -0.54249 0.443329 -0.24384 | |
Cla.coc 0.1998745 -0.11892 0.05746 -0.11248 0.073434 0.09607 | |
Cla.cor -0.3377207 0.00630 -0.30418 -0.13082 0.007785 -0.09738 | |
Cla.gra -0.0591848 -0.14946 -0.27214 -0.18229 0.113776 -0.19158 | |
Cla.fim -0.0005966 -0.11638 0.03219 -0.27266 -0.185276 0.01141 | |
Cla.cri -0.2353142 -0.13014 0.04825 -0.44966 -0.082457 -0.30900 | |
Cla.chl 0.2960198 0.43031 -0.44880 -0.45712 -0.026098 -0.32614 | |
Cla.bot -0.6121890 -0.67705 -0.17531 -1.05814 -0.701290 -0.74162 | |
Cla.ama 0.0339234 -0.89659 -1.10581 -0.06753 0.078437 0.24184 | |
Cla.sp 0.6893702 0.73032 0.49452 -0.39382 0.126202 -0.21620 | |
Cet.eri 0.2703176 0.10341 -0.30842 -0.06482 0.593267 -0.44989 | |
Cet.isl 0.1448207 0.10344 -0.31552 -0.70305 -0.482515 -0.22317 | |
Cet.niv 1.3069886 -0.01489 0.54845 1.61791 -1.099398 -2.15267 | |
Nep.arc -0.8040582 0.39185 -0.06934 -0.02375 -0.746439 1.04971 | |
Ste.sp 0.2935602 -1.44392 -0.78477 0.79952 0.694834 0.72962 | |
Pel.aph -0.6296777 0.05166 -0.34405 -0.15437 -0.058790 -0.21384 | |
Ich.eri 0.3228067 -1.83898 -0.44899 0.64843 0.595119 0.78301 | |
Cla.cer 0.9088477 0.22411 0.50813 1.00260 -0.582433 -0.84668 | |
Cla.def -0.3460925 -0.21145 -0.11090 -0.54258 0.007175 -0.33724 | |
Cla.phy 0.7223026 1.12784 -0.05001 -0.23188 0.099363 0.06092 | |
Site scores (weighted averages of species scores) | |
CCA1 CCA2 CCA3 CCA4 CCA5 CCA6 | |
18 0.2368 -1.0455 -0.79083 -0.149031 -0.49251 -0.35212 | |
15 -0.9799 -0.2750 0.34407 0.111139 0.63356 0.30849 | |
24 -1.0983 0.8478 -2.51016 -0.080535 4.40120 -2.69293 | |
27 -1.6399 0.4846 0.31083 1.033926 -0.68668 0.16045 | |
23 -0.6573 -0.3813 -0.70171 -0.837942 -0.50965 -0.35934 | |
19 -0.2220 0.6886 -0.03737 -0.002568 -0.65742 0.07187 | |
22 -1.1371 -0.7562 1.68441 -1.251373 0.68408 1.82825 | |
16 -0.7322 -0.9683 1.38438 -0.848066 1.09474 1.52365 | |
28 -2.4224 0.8605 0.77192 2.459293 -1.07010 0.02053 | |
13 0.5930 -1.4057 2.91790 -0.312366 1.59649 -2.08999 | |
14 -0.3296 -0.8773 0.53603 -1.974878 1.81909 -0.90880 | |
20 -0.5440 -0.3236 -0.58922 -0.198890 0.30072 -0.50529 | |
25 -1.2792 0.4454 -1.17274 -0.360068 1.82086 0.33613 | |
7 0.7264 -1.9319 -0.98918 1.155440 -0.49232 -0.08616 | |
5 0.9323 -2.3201 -1.39730 2.072908 0.83123 1.69279 | |
6 0.7454 -1.2134 -0.56548 0.372969 -0.26442 -0.15445 | |
3 1.3118 0.5893 -0.06894 0.401225 -0.39972 0.94502 | |
4 1.3726 -0.1616 0.83676 1.878597 -1.28329 -3.05924 | |
2 1.3068 1.3075 0.11566 -0.055773 -0.47463 1.05104 | |
9 1.1513 1.9030 0.25604 -0.576516 -0.64079 0.50208 | |
12 0.9003 1.2149 0.03147 -0.437409 -0.44022 0.54418 | |
10 1.2566 1.7341 0.25151 -0.507033 -0.60207 0.74947 | |
11 0.4809 0.1232 -0.01664 0.339108 0.09187 0.24062 | |
21 -0.8258 -0.3306 -1.27405 -2.712570 -3.96294 -1.33584 | |
Site constraints (linear combinations of constraining variables) | |
CCA1 CCA2 CCA3 CCA4 CCA5 CCA6 | |
18 -0.51001 -0.86277 -1.265868 -0.84832 0.00585 -0.55362 | |
15 -0.11359 0.47962 0.292164 -0.71983 -1.13275 -0.12336 | |
24 -0.22365 0.90898 -2.201280 0.15538 2.96165 -1.67268 | |
27 -1.64472 0.76232 0.420020 0.74263 -0.06008 0.22434 | |
23 -1.32539 -0.30282 -0.919558 -0.37197 0.23629 -0.78394 | |
19 -1.07981 0.11303 -0.113499 -0.60422 -0.19793 -1.29585 | |
22 -0.80792 -0.42650 1.084574 -0.98064 1.21790 1.50140 | |
16 -0.09523 -1.62392 0.407793 -0.06884 -0.07282 0.33619 | |
28 -2.23615 0.64537 0.663728 2.48585 -0.46791 0.25333 | |
13 0.42015 -1.10098 3.235920 -0.54956 1.29688 -1.38806 | |
14 -0.14558 -0.43109 0.310536 -0.84389 0.19863 -0.03026 | |
20 -0.46785 0.19132 -0.309976 -1.55804 -0.35312 -0.83013 | |
25 -0.94343 0.48338 -0.020181 -0.08962 -0.75201 1.14075 | |
7 1.13410 -1.71592 -0.683778 1.28206 -0.45511 -0.70628 | |
5 0.46572 -2.30802 -1.130203 1.25890 1.45704 1.70523 | |
6 0.86525 0.07408 -0.448437 -0.03867 -0.80286 1.09713 | |
3 1.05262 0.42467 -0.654573 0.68815 -1.10660 0.89967 | |
4 1.39514 -0.07478 0.728546 1.89627 -1.33345 -2.59477 | |
2 1.33321 0.54940 0.492977 0.24130 0.78232 0.74187 | |
9 1.29092 1.95150 0.005342 -0.81565 0.26355 0.18074 | |
12 0.41778 0.20237 0.558481 -0.57975 -0.17713 0.78064 | |
10 0.48239 1.37874 -0.125838 0.29170 0.38215 -0.13758 | |
11 0.56717 0.36508 -0.101642 -0.04929 -0.36747 0.15785 | |
21 -0.55130 -1.11370 -0.928444 -1.85875 -1.87942 -0.58312 | |
Biplot scores for constraining variables | |
CCA1 CCA2 CCA3 CCA4 CCA5 CCA6 | |
Baresoil -0.52916 -0.39394 -0.08617 -0.48990 -0.17135 -0.47822 | |
Humdepth -0.74502 0.09330 0.17115 -0.23030 -0.12889 -0.16495 | |
pH 0.50738 0.14614 -0.26885 0.17600 -0.10593 0.17747 | |
N -0.18023 -0.58693 0.04837 0.36820 0.15022 0.16997 | |
P -0.36138 0.63661 0.04617 0.32849 0.17792 -0.38565 | |
K -0.39232 0.31726 0.55953 0.12491 0.25046 -0.48506 | |
Ca -0.48559 0.41273 0.01284 -0.06856 0.28471 -0.24271 | |
Mg -0.45563 0.35145 -0.07675 -0.07944 0.47484 -0.50687 | |
S -0.03901 0.50851 0.36649 0.02615 0.41656 -0.63216 | |
Al 0.80742 0.10327 0.25002 0.22841 -0.03898 -0.35763 | |
Fe 0.67570 0.02563 0.09560 0.24078 -0.08059 -0.04175 |
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Thank you for this code.
I am applying this to my data, but I don't get the OTUs and sites names. Any idea on how to resolve this ?
Thank you,
Najoua