A snippet that demonstrates using PyLibTiff to open a .tiff file containing a 2-color image stack

tiff.py

import numpy as np
from libtiff import TIFFfile
from libtiff import TIFF

def read(fileName):
    """
    Script to import tif file from imageJ,
    usage: zstack =  tiff.read(inFileName)

    PTW 2015/01/29
    """
    tiff = TIFFfile(fileName)
    samples, sample_names = tiff.get_samples()
    
    outList = []
    for sample in samples:
        outList.append(np.copy(sample)[...,np.newaxis])
        
    out = np.concatenate(outList,axis=-1)
    out = np.rollaxis(out,0,3)
    out = np.flipud(out)
    
    tiff.close()
    
    return out

def write_stack(fileName, array, compression=None):
    """
    Script to export tif file to imageJ,
    usage: tiff.write_stack(outFileName, array, compression=None)

    PTW 2015/04/22
    """
    #NEED: to document and decide about dealing with array with fewer dimensions than 4...
    outTiff = TIFF.open(fileName, mode='w')
    a = np.flipud(array)
    a = np.rollaxis(a, 3, 0)
    
    for zInd in range(a.shape[3]):
        outTiff.write_image(a[:,:,:,zInd],compression=compression, write_rgb=True)
        
    outTiff.close()
    
    return None