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UCSC narrowPeak to bigBed
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#!/bin/bash | |
# set the path to as file for narrowPeak. Locate it in your UCSC source code directory or google... | |
asfile=/data/db/UCSC_sqls/encode/narrowPeak.as | |
which bedToBigBed &>/dev/null || { echo "bedToBigBed not found! Download: <http://hgdownload.cse.ucsc.edu/admin/exe/>"; exit 1; } | |
if [ $# -lt 2 ];then | |
echo "Need 2 parameters! <narrowPeak> <chrom info>" | |
exit | |
fi | |
F=$1 | |
G=$2 | |
bedtools slop -i ${F} -g ${G} -b 0 | bedClip stdin ${G} ${F}.clip | |
# fix scores over 1000 | |
perl -pi -e 'chomp;@_=split;if ($_[4]>1000) {$_[4]=1000} $_=join("\t",@_)."\n"' ${F}.clip | |
bedToBigBed -type=bed6+4 -as=${asfile} ${F}.clip $G ${F/.narrowPeak/}.bb | |
rm -f ${F}.clip |
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