Make plots about genome sequencing, size, and gene content

genome_plots.r

source("http://bioconductor.org/biocLite.R")
biocLite("genomes")

library(genomes)
library(ggplot2)

valid <- c("released", "created", "submitted")

data(proks)
update(proks)
pn <- which(names(proks) %in% valid)[1]
pt <- table(c(proks[, pn], Sys.Date()))
pd <- data.frame("date" = as.Date(names(pt)), "genomes"=as.numeric(pt))
pd$clade = 'prokaryotes'

data(euks)
update(euks)
en <- which(names(euks) %in% valid)[1]
et <- table(c(euks[, en], Sys.Date()))
ed <- data.frame("date" = as.Date(names(et)), "genomes"=as.numeric(et))
ed$clade = 'eukaryotes'

data(virus)
update(virus)
vn <- which(names(virus) %in% valid)[1]
vt <- table(c(virus[, vn], Sys.Date()))
vd <- data.frame("date" = as.Date(names(vt)), "genomes"=as.numeric(vt))
vd$clade = 'viruses'

theme_set(theme_gray(base_size = 28))

# plots of the number of sequenced genomes accumulating over time
ggplot(vd, aes(x = date, y=cumsum(genomes))) + geom_line(size = 3, colour='red') + xlab("Year") + ylab("Number of sequenced genomes") + ggtitle("Viruses")
ggplot(ed, aes(x = date, y=cumsum(genomes))) + geom_line(size = 3, colour='red') + xlab("Year") + ylab("Number of sequenced genomes") + ggtitle("Eukaryotes")
ggplot(pd, aes(x = date, y=cumsum(genomes))) + geom_line(size = 3, colour='red') + xlab("Year") + ylab("Number of sequenced genomes") + ggtitle("Prokaryotes")

# plots of genome size against number of protein coding genes
ggplot(euks, aes(x = proteins, y = size)) + geom_point(alpha = 0.5, size=4) + ylim(c(0,3500)) + xlim(c(0,70000)) + ggtitle("Eukaryotes") + xlab("Number of protein coding genes") + ylab("Genome size (MB)")
ggplot(proks, aes(x = proteins, y = size)) + geom_point(alpha = 0.33, size=4) + ylim(c(0,17)) + ggtitle("Prokaryotes") + xlab("Number of protein coding genes") + ylab("Genome size (MB)")
ggplot(virus, aes(x = proteins, y = size)) + geom_point(alpha = 0.33, size=4)  + ylim(c(0,300)) + xlim(c(0,400)) + ggtitle("Viruses") + xlab("Number of protein coding genes") + ylab("Genome size (MB)")


# to answer a twitter question (https://twitter.com/potatodoctor/status/630281782315909120), we need to look more closely at animals
ggplot(euks[which(euks$group=="Animals"),], aes(x = proteins, y = size)) + geom_point(alpha = 0.95, size=4, aes(colour=subgroup)) + ylim(c(0,3500)) + xlim(c(0,70000)) + ggtitle("Animals") + xlab("Number of protein coding genes") + ylab("Genome size (MB)") + scale_colour_brewer(palette="Set1")

# another question was what the R-squared on the Prokaryote plot is (https://twitter.com/razibkhan/status/630278982521323520)
# note that to do this properly we'd need to control for non-independence due to relatedness, but we can get a rough idea like this
m1 = lm(proks$size~proks$genes)
summary(m1)

# that R-squared looks high for the plot. However, if you re-draw the plot with smaller points, you can see that most of the points cluster RIGHT on the line
ggplot(proks, aes(x = proteins, y = size)) + geom_point(alpha = 0.05, size=1.0) + ylim(c(0,17)) + ggtitle("Prokaryotes") + xlab("Number of protein coding genes") + ylab("Genome size (MB)")

If you notice an issue, have a question, or can think of a way to improve the plots (or the underlying data), please post below!

Not an R spesialist, but I am getting this error:

data(proks)
Warning message:
In data(proks) : data set 'proks' not found

What am I missing?