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scientificprotocols/protocol.md

Last active August 29, 2015 14:03
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CHROME GELATINISED SLIDES AND COVERSLIPS
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protocol.md 
Author: University of Nottingham Medical School Division of Histopathology

Method:

  1. Dissolve 5g of gelatine in 1 litre of distilled water. Do not heat over 60C.
  • When the gelatine has dissolved add 0.5g of chrome alum (chromic potassium sulphate).
  • Cool and dip racks of slides or coverslips into the solution.
  • Drain slides and coverslips onto paper tissue.
  • Dry overnight in a 37C incubator.

Notes:

The solution ON USE has to be warm but not hot. It can be kept in a refrigerator overnight and re-warmed on a hot plate so that the method can be repeated.

DOI

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