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scientificprotocols/protocol.md

Last active August 29, 2015 14:03
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3-AMINOPROPYLTRIETHOXYSILANE TREATED SLIDES
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protocol.md 
Author: University of Nottingham Medical School Division of Histopathy

METHOD:

  1. Wash glass slides in detergent for 30 minutes.

  • Wash glass slides in running tap water for 30 minutes.

  • Wash glass slides in distilled water 2x5 minutes.

  • Wash glass slides in 95% alcohol 2x5 minutes.

  • Air dry for 10 minutes.

  • Immerse slides in a freshly prepared 2% solution for 3-aminopropyltriethoxysilane in dry acetone for 5 seconds.

  • Wash briefly in distilled water twice.

  • Dry overnight at 42C and store at room temperature.

    • Note: 300mls of silane solution is sufficient to do 200 slides. Treated slides can be kept indefinitely.

DOI

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